13
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Long-term proliferation in culture and germline transmission of mouse male germline stem cells.

      Biology of reproduction

      Animals, Newborn, Stem Cells, Stem Cell Transplantation, transplantation, Spermatogonia, physiology, Seminiferous Tubules, Phenotype, Mice, Inbred DBA, Mice, Male, therapy, physiopathology, Infertility, Male, Germ Cells, Flow Cytometry, Fertilization in Vitro, Fertility, Cells, Cultured, Cell Division, Animals

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Spermatogenesis is a complex process that originates in a small population of spermatogonial stem cells. Here we report the in vitro culture of spermatogonial stem cells that proliferate for long periods of time. In the presence of glial cell line-derived neurotrophic factor, epidermal growth factor, basic fibroblast growth factor, and leukemia inhibitory factor, gonocytes isolated from neonatal mouse testis proliferated over a 5-month period (>10(14)-fold) and restored fertility to congenitally infertile recipient mice following transplantation into seminiferous tubules. Long-term spermatogonial stem cell culture will be useful for studying spermatogenesis mechanism and has important implications for developing new technology in transgenesis or medicine.

          Related collections

          Author and article information

          Journal
          12700182
          10.1095/biolreprod.103.017012

          Comments

          Comment on this article