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      • Record: found
      • Abstract: found
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      A virtual burrow assay for head–fixed mice measures habituation, discrimination, exploration and avoidance without training

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          Abstract

          We have designed an assay that measures approach and avoidance behaviors in head-fixed mice at millisecond timescale, is compatible with standard electrophysiological and optical methods for measuring neuronal activity, and requires no training. The Virtual Burrow Assay simulates a scenario in which a mouse, poised at the threshold of its burrow, evaluates whether to exit the enclosure or to retreat inside. The assay provides a sensitive readout of habituation, discrimination and exploration, as well as avoidance of both conditioned and innately aversive cues.

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          Functional imaging of hippocampal place cells at cellular resolution during virtual navigation

          Daniel A. Dombeck, Christopher D. Harvey, Lin Tian … (2010)
          Spatial navigation is a widely employed behavior in rodent studies of neuronal circuits underlying cognition, learning and memory. In vivo microscopy combined with genetically-encoded indicators provides important new tools to study neuronal circuits, but has been technically difficult to apply during navigation. We describe methods to image the activity of hippocampal CA1 neurons with sub-cellular resolution in behaving mice. Neurons expressing the genetically encoded calcium indicator GCaMP3 were imaged through a chronic hippocampal window. Head-fixed mice performed spatial behaviors within a setup combining a virtual reality system and a custom built two-photon microscope. Populations of place cells were optically identified, and the correlation between the location of their place fields in the virtual environment and their anatomical location in the local circuit was measured. The combination of virtual reality and high-resolution functional imaging should allow for a new generation of studies to probe neuronal circuit dynamics during behavior.
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            Habituation: A model phenomenon for the study of neuronal substrates of behavior.

            William Spencer, Richard Thompson (1966)
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              Habituation: a dual-process theory.

              Philip Groves, Richard Thompson (1970)
              Article 0 comments Cited 207 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                Andrew JP Fink:
                ORCID: http://orcid.org/0000-0003-4191-3298
                Carl E Schoonover:
                ORCID: https://orcid.org/0000-0002-6397-1010
                Peggy Mason: Role: Reviewing Editor
                Catherine Dulac: Role: Senior Editor
                Journal
                Journal ID (nlm-ta): eLife
                Journal ID (iso-abbrev): Elife
                Journal ID (publisher-id): eLife
                Title: eLife
                Publisher: eLife Sciences Publications, Ltd
                ISSN (Electronic): 2050-084X
                Publication date (Electronic, pub): 17 April 2019
                Publication date Collection: 2019
                Volume: 8
                Electronic Location Identifier: e45658
                Affiliations
                [1 ]deptDepartment of Neuroscience Howard Hughes Medical Institute, Columbia University New YorkUnited States
                University of Chicago United States
                Harvard University United States
                University of Chicago United States
                Harvard University United States
                Humboldt University of Berlin Germany
                Author notes
                [†]

                These authors contributed equally to this work.

                Author information
                http://orcid.org/0000-0003-4191-3298
                http://orcid.org/0000-0002-3141-4076
                https://orcid.org/0000-0002-6397-1010
                Article
                Publisher ID: 45658
                DOI: 10.7554/eLife.45658
                PMC ID: 6469927
                PubMed ID: 30994457
                SO-VID: d8260298-8045-4ef0-8008-c5b84d7eadc5
                Copyright © © 2019, Fink et al
                License:

                This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

                History
                Date received : 30 January 2019
                Date accepted : 28 March 2019
                Funding
                Funded by: FundRef http://dx.doi.org/10.13039/100000011, Howard Hughes Medical Institute;
                Award Recipient :
                Funded by: FundRef http://dx.doi.org/10.13039/100005237, Helen Hay Whitney Foundation;
                Award Recipient :
                The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
                Categories
                Subject: Tools and Resources
                Subject: Neuroscience
                Custom metadata
                Author impact statement A naturalistic assay for head-fixed mice permits observation of a diverse set of innate and learned behaviors without requiring any training.

                ScienceOpen disciplines: Life sciences
                Keywords: virtual burrow assay,habituation,fear conditioning,sensory discrimination,exploration,head-fixed behavior,mouse
                Data availability:
                ScienceOpen disciplines: Life sciences
                Keywords: virtual burrow assay, habituation, fear conditioning, sensory discrimination, exploration, head-fixed behavior, mouse

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