Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida

Abstract

Potato cyst nematodes (PCNs), Globodera rostochiensis and G. pallida, cause important economic losses. They are hard to manage because of their ability to remain dormant in soil for many years. Although general knowledge about these plant parasitic nematodes has considerably increased over the past decades, very little is known about molecular events involved in cyst dormancy and hatching, two key steps of their development. Here, we have studied the progression of PCN transcriptomes from dry cysts to hatched juveniles using RNA-Seq. We found that several cell detoxification-related genes were highly active in the dry cysts. Many genes linked to an increase of calcium and water uptake were up-regulated during transition from dormancy to hydration. Exposure of hydrated cysts to host plant root exudates resulted in different transcriptional response between species. After 48 h of exposure, G. pallida cysts showed no significant modulation of gene expression while G. rostochiensis had 278 differentially expressed genes. The first G. rostochiensis significantly up-regulated gene was observed after 8 h and was coding for a transmembrane metalloprotease. This enzyme is able to activate/inactivate peptide hormones and could be involved in a cascade of events leading to hatching. Several known effector genes were also up-regulated during hatching.

Contributors

Benjamin Mimee: [e-mail]

Journal

Journal ID (nlm-ta): Sci Rep

Journal ID (iso-abbrev): Sci Rep

Title: Scientific Reports

Publisher: Nature Publishing Group UK (London )

ISSN (Electronic): 2045-2322

Publication date (Electronic): 20 June 2017

Publication date PMC-release: 20 June 2017

Publication date Collection: 2017

Volume: 7

Affiliations

[1 ]ISNI 0000 0001 1302 4958, GRID grid.55614.33, , Agriculture and Agri-Food Canada, ; 430, Boulevard Gouin Saint-Jean-sur-Richelieu (Québec), J3B 3E6 Québec, Canada

[2 ]ISNI 0000 0000 9064 6198, GRID grid.86715.3d, Département de Biologie, , Université de Sherbrooke, ; Sherbrooke, J1K 2R1 Canada

[3 ]ISNI 0000 0001 1014 6626, GRID grid.43641.34, Cell and Molecular Sciences, , The James Hutton Institute, Invergowrie, ; Dundee, DD2 5DA United Kingdom

[4 ]Canadian Food Inspection Agency, Ottawa Laboratory Fallowfield (OLF), 3851 Fallowfield Road, Ottawa, Ontario K2H 8P9 Canada

[5 ]ISNI 0000 0001 2183 4846, GRID grid.4711.3, Institute for Sustainable Agriculture (IAS), , Spanish National Research Council (CSIC), ; Avenida Menéndez Pidal s/n, 14004 Córdoba, Campus de Excelencia Internacional Agroalimentario ceiA3 Spain

Article

Publisher ID: 3871

DOI: 10.1038/s41598-017-03871-x

PMC ID: 5478601

PubMed ID: 28634407

Copyright statement: © The Author(s) 2017

License:

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Categories

Subject: Article

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issue-copyright-statement © The Author(s) 2017