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      Fine mapping and characterization of Sr21, a temperature-sensitive diploid wheat resistance gene effective against the Puccinia graminis f. sp. tritici Ug99 race group

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          Abstract

          A race of Puccinia graminis f. sp. tritici, the causal pathogen of stem rust of wheat, known as Ug99, and its variants, are virulent to plants carrying stem rust resistance genes currently deployed in most wheat cultivars worldwide. Therefore, identification, mapping and deployment of effective resistance genes are critical to reduce this threat. Resistance gene Sr21 identified in diploid wheat T. monococcum can be effective against races from the Ug99 race group, but both susceptible and partial resistant reactions have been reported in previous studies. To clarify this conflicting information we screened four monogenic lines with Sr21 and four susceptible controls with 16 Pgt isolates including 5 isolates of the Ug99 race group under three different temperatures and three different photoperiods. We observed that temperature influences the interaction between monogenic lines with Sr21 and Ug99 race group isolates, and may be one source of previous inconsistencies. This result indicates that, although Sr21 confers partial resistance against Ug99, its effectiveness can be modulated by environmental conditions and should not be deployed alone. Using two large diploid wheat-mapping populations (total 3,788 F 2 plants) we mapped Sr21 approximately 50 cM from the centromere on the long arm of chromosome 2A m within a 0.20 cM interval flanked by sequence-based markers FD527726 and EX594406. The closely linked markers identified in this study will be useful to reduce the T. monococcum segments introgressed into common wheat, accelerate Sr21 deployment in wheat breeding programs, and facilitate the map-based cloning of this gene.

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          Author and article information

          Journal
          0145600
          8295
          Theor Appl Genet
          Theor. Appl. Genet.
          TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
          0040-5752
          1432-2242
          26 October 2016
          30 January 2015
          April 2015
          30 October 2016
          : 128
          : 4
          : 645-656
          Affiliations
          [1 ]Department of Plant Sciences, University of California, Davis, CA95616
          [2 ]Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China
          [3 ]USDA-ARS Cereal Disease Laboratory and Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108
          [4 ]Howard Hughes Medical Institute, Chevy Chase, MD 20815
          [5 ]Kansas State University, Dept. Plant Pathology, Manhattan, KS 66506
          Author notes
          [* ]Corresponding author: J. Dubcovsky, jdubcovsky@ 123456ucdavis.edu . Phone: (530) 752 5159

          The first three authors contributed equally to this work.

          Article
          PMC5086425 PMC5086425 5086425 hhmipa825318
          10.1007/s00122-015-2460-x
          5086425
          25634104
          d83a8502-8ec6-4767-a841-fadb07e3b26e
          History
          Categories
          Article

          Ug99,resistance gene,diploid wheat,genetic map,stem rust
          Ug99, resistance gene, diploid wheat, genetic map, stem rust

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