A sensitive and accurate quantification method for the detection of hepatitis B virus covalently closed circular DNA by the application of a droplet digital polymerase chain reaction amplification system.

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Abstract

To develop a sensitive and accurate assay system for the quantification of covalently closed circular HBV DNA (cccDNA) for future clinical monitoring of cccDNA fluctuation during antiviral therapy in the liver of infected patients.

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Journal ID (iso-abbrev): Biotechnol. Lett.

Title: Biotechnology letters

Publisher: Springer Nature

ISSN (Electronic): 1573-6776

ISSN (Print): 0141-5492

Publication date (Electronic): Oct 2015

Volume: 37

Issue: 10

Affiliations

[1 ] Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education, and The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, People's Republic of China. mudi0452@163.com.

[2 ] Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education, and The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, People's Republic of China. yanyan_5212@126.com.

[3 ] Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education, and The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, People's Republic of China. tangtanghui@gmail.com.

[4 ] Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing Medical University, Yixueyuan Rd #1, Yuzhong District, P.O. Box #149, Chongqing, 400010, People's Republic of China. yongliao@cqmu.edu.cn.

Article

Publisher Item ID: 10.1007/s10529-015-1890-5

DOI: 10.1007/s10529-015-1890-5

PubMed ID: 26187315

SO-VID: d8408148-3a99-42d6-973d-9a3c9cd06a3b

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Keywords: Covalently closed circular DNA (cccDNA),Droplet digital PCR,HepG2.215 cell line,Hepatitis B virus,Plasmid-safe ATP-dependent deoxynuclease,cccDNA-specific primer

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Keywords: Covalently closed circular DNA (cccDNA), Droplet digital PCR, HepG2.215 cell line, Hepatitis B virus, Plasmid-safe ATP-dependent deoxynuclease, cccDNA-specific primer

A sensitive and accurate quantification method for the detection of hepatitis B virus covalently closed circular DNA by the application of a droplet digital polymerase chain reaction amplification system.

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