Objective: The aim of this study was to quantify the frequency of burnout among Slovak Nurses to shed more light on the associations between the workload and burnout syndrome. Design: cross-sectional design. Participants and methods: A total of 770 Nurses from Slovakia participated in the study. A structured questionnaire with work-related items and Maslach Burnout Inventory were used to collect the data. Descriptive statistics, t-test, and analyses of variance (ANOVA) were calculated. Results: Statistically significantly (p ≤ 0.05) higher degree of burnout syndrome were identified in Nurses with longer work experience; employed on oncology departments; intensive care units; working in 8 or 12 hour shifts compared with no shifts. Conclusion: Research results suggest that the profession of Nurses should be supported by management aimed at preventing work-related stress in Clinical Practice.