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Diversidad de frijoles nativos de diferentes regiones del estado de Puebla Translated title: Native beans diversity of different regions from state of Puebla

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      Abstract

      El frijol común representa un cultivo básico en México con una gran variabilidad en sus características morfológicas. En estudios sobre la diversidad genética se han usado marcadores moleculares, entre los que se encuentran los RAPD. El presente trabajo tuvo como objetivo caracterizar mediante caracteres morfológicos, agronómicos, de calidad y marcadores RAPD, variedades de frijoles nativos cultivadas en diferentes comunidades del estado de Puebla. La extracción de ADN se realizó en una muestra de hojas jóvenes de las variedades sembradas bajo invernadero. Se utilizaron 7 iniciadores RAPDs de la serie OPERON Technologies. Las bandas polimórficas se codificaron por presencia y ausencia. Con el programa NTSYS pc 2.2 se generó un dendograma empleando el índice de similitud de Dice, con nivel de corte a un valor de 0.48, donde se definieron 13 grupos. Seis grupos estuvieron integrados por una variedad, 4 grupos por 2 variedades, 2 grupos por 3 variedades y un grupo formado por 7 variedades. Los grupos definidos se integraron por colores de grano distintos y coincidieron en otras características morfológicas como días a floración, tiempo de cocción y contenido de proteína.

      Translated abstract

      Common bean is basic crop in Mexico with great variability in its morphological characteristics. RAPD is among molecular markers, which have been used in studies about genetic diversity. The aim of this work was to characterize by morphological, agronomical, quality characters and RAPD markers the varieties harvested in different localities from state of Puebla. DNA extraction was made from sample of young leaves of varieties planted under greenhouse. 7 RAPDs starters from OPERON Technologies were used. Polymorphical bands were coded by presence and absence. With NTSYS pc 2.2 software dendrogram was created using Dice's similarity index, with cut level at value of 0.48 where 13 groups were defined. Six groups were comprised by single variety, 4 groups by 2 varieties, 2 groups by 3 varieties and one group by 7 varieties. The defined groups were comprised by different grain colors and coincided in other morphological characteristics like days to flowering, cooking time and protein content.

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          Linkage among isozyme, RFLP and RAPD markers in Vicia faba.

          Segregating allozyme and DNA polymorphisms were used to construct a preliminary linkage map for faba bean. Two F2 populations were analyzed, the most informative of which was segregating for 66 markers. Eleven independently assorting linkage groups were identified in this population. One of the groups contained the 45s ribosomal array and could be assigned to the large metacentric chromosome I on which the nucleolar organizer region is located. This linkage group also contained two isozyme loci, Est and Tpi-p, suggesting that it may share some homology with chromosome 4 of garden pea on which three similar markers are syntenic. Additional aspects of the map and the extent of coverage of the total nuclear genome are discussed.
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            Author and article information

            Affiliations
            [1 ] Colegio de Postgraduados Mexico
            [2 ] Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias Mexico
            [3 ] Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias Mexico
            Contributors
            Role: ND
            Role: ND
            Role: ND
            Role: ND
            Role: ND
            Journal
            remexca
            Revista mexicana de ciencias agrícolas
            Rev. Mex. Cienc. Agríc
            Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (Texcoco )
            2007-0934
            June 2012
            : 3
            : 3
            : 467-480
            S2007-09342012000300005

            http://creativecommons.org/licenses/by/4.0/

            Product
            Product Information: SciELO Mexico
            Categories
            Agriculture, Multidisciplinary

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