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      Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization.

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          Abstract

          The association of genes with the nuclear pore complex (NPC) and nuclear transport factors has been implicated in transcriptional regulation. We therefore examined the association of components of the nuclear transport machinery including karyopherins, nucleoporins, and the Ran guanine-nucleotide exchange factor (RanGEF) with the Saccharomyces cerevisiae genome. We find that most nucleoporins and karyopherins preferentially associate with a subset of highly transcribed genes and with genes that possess Rap1 binding sites whereas the RanGEF preferentially associates with transcriptionally inactive genes. Consistent with coupling of transcription to the nuclear pore, we show that transcriptional activation of the GAL genes results in their association with nuclear pore proteins, relocation to the nuclear periphery, and loss of RanGEF association. Taken together, these results indicate that the organization of the genome is coupled via transcriptional state to the nuclear transport machinery.

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          Author and article information

          Journal
          Cell
          Cell
          Elsevier BV
          0092-8674
          0092-8674
          May 14 2004
          : 117
          : 4
          Affiliations
          [1 ] Department of Systems Biology, Harvard Medical School and Department of Cancer Biology, The Dana-Farber Cancer Institute, Boston, MA 02115, USA.
          Article
          S0092867404004489
          10.1016/s0092-8674(04)00448-9
          15137937
          d8ac5669-f076-4b08-b00c-40a448b03a55
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