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      Effects of Moist-Chamber and McCarey-Kaufman Medium Storage on the Metabolic Status of the Cornea: A 31P-Magnetic Resonance Analysis

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          Abstract

          The rate of change in concentration of corneal phosphatic metabolites of cat corneas stored in moist chamber and McCarey-Kaufman (M-K) medium was determined in order to provide a basis for prediction of the corneal metabolic status at a given storage time. Perchloric acid corneal extracts were examined by phosphorus-31 magnetic resonance after storage at 4 °C of whole globes under moist-chamber conditions up to 48 h and of excised corneas in M-K medium up to 168 h. A significant decline in the corneal concentrations of ATP and a significant increase in inorganic phosphate occurred for both storage methods; however, depending on the metabolite, the rate of decline or increase was significantly greater for the moist-chamber-stored corneas. The phosphorylated sugars significantly increased and the glycerophosphodiesters significantly decreased in the moist-chamber-stored corneas, whereas both metabolites remained unchanged in the M-K-medium-stored corneas. There was no significant change in the dinucleotides and nucleoside diphospho-sugars during the time course fot both storage methods. A threefold greater rate of decline was noted in the tissue energy modulus for the moist-chamber-stored corneas than for the M-K-medium-stored corneas (-0.0465 vs. -0.0121 modulus values/h). M-K medium was significantly more effective in the maintenance of high-energy phosphatic metabolites. The mathematical model for these rate determinations provides a basis for prediction of the corneal metabolic status at a given time in moist-chamber or M-K medium storage.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1988
          1988
          10 December 2009
          : 20
          : 6
          : 368-375
          Affiliations
          aDivision of Ophthalmology, Case Western Reserve University, Cleveland, Ohio; bHowe Laboratory of Ophthalmology, Harvard Medical School; and Massachusetss Eye and Ear Infirmary, Boston, Mass.; cMagnetic Resonance Laboratory, Chicago College of Osteopathic Medicine, Chicago, Ill., USA
          Article
          266754 Ophthalmic Res 1988;20:368–375
          10.1159/000266754
          3237395
          © 1988 S. Karger AG, Basel

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          Page count
          Pages: 8
          Categories
          Original Paper

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