Porcine skin as a source of biodegradable matrices: alkaline treatment and glutaraldehyde crosslinking

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Abstract

In this work, the modifications promoted by alkaline hydrolysis and glutaraldehyde (GA) crosslinking on type I collagen found in porcine skin have been studied. Collagen matrices were obtained from the alkaline hydrolysis of porcine skin, with subsequent GA crosslinking in different concentrations and reaction times. The elastin content determination showed that independent of the treatment, elastin was present in the matrices. Results obtained from in vitro trypsin degradation indicated that with the increase of GA concentration and reaction time, the degradation rate decreased. From thermogravimetry and differential scanning calorimetry analysis it can be observed that the collagen in the matrices becomes more resistant to thermal degradation as a consequence of the increasing crosslink degree. Scanning electron microscopy analysis indicated that after the GA crosslinking, collagen fibers become more organized and well-defined. Therefore, the preparations of porcine skin matrices with different degradation rates, which can be used in soft tissue reconstruction, are viable.

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Biodegradation of PLA/GA polymers: increasing complexity.

Michel Vert, Jacques Mauduit, Suming Li (1994)

The degradation of aliphatic polyesters derived from lactic and glycolic acids (PLA/GA) depends on many factors. It has been found recently that the interior of large size devices degrades faster than the outer zone. A qualitative model has been proposed to account for this heterogeneous degradation. It is based on diffusion-reaction phenomena combined with the well-known autocatalytic effect of carboxylic chain ends. This contribution recalls the present understanding of the hydrolytic degradation of PLA/GA polymers and emphasizes its complexity on the basis of the influence of secondary factors such as the presence of a basic load, namely, gentamycin, in poly(lactic acid) matrices, and the presence of long stereoregular sequences in poly(DL-lactic acid) macromolecules.