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      Effects of Buflomedil and Pentoxifylline on Hamster Skin-Flap Microcirculation: Prediction of Flap Viability Using Orthogonal Polarization Spectral Imaging

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          Abstract

          OBJECTIVE

          This study investigated the effects of buflomedil and pentoxifylline, both of which are used in reconstructive surgery of hamster skin flap microcirculation, and evaluated the skin flap survival rate by orthogonal polarization spectral imaging.

          METHOD

          Twenty-four adult male Syrian golden hamsters were divided into three groups: a control (C, 0.1 ml 0.9% saline), buflomedil (B, 3 mg/kg/day), and pentoxifylline group (P, 14.5 mg/kg/day). Treatments administered intraperitoneally were initiated 1 hour before skin flap preparation and continued for 7 days post-operatively at 12-hour intervals. Preparations (skin flaps) were divided into 12 fields, which were organized into six bands. Functional capillary density (FCD, in mm/mm 2), distance from the skin flap base to blood flow cessation (Dist with flow, in cm), percentage of viable skin (VA, in%), and qualitative analysis of blood flow by orthogonal polarization spectral imaging were performed at 1 and 24 hours and on the seventh post-operative day.

          RESULT

          Bands IV, V, and VI presented no flow independent of time. The functional capillary density group B was higher than that of groups C and P, primarily after 24 hours. All groups showed an increase in D with time but reached similar final distances (C = 2.73, B = 2.78 and P = 2.70 cm). Moreover, the percentage of viable areas remained at approximately 50%. The orthogonal polarization spectral imaging was useful to assess viability by counting fields with and without blood flow.

          CONCLUSIONS

          Functional capillary density values were higher in the buflomedil group compared to the control and pentoxifylline groups in this model. Functional capillary density did not influence D or the percentage of VA, and the technique showed favorable potential to assess/predict the viability of skin flaps within 1 h after surgery.

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          Most cited references100

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          THE DESIGN OF A PEDICLE FLAP IN THE RAT TO STUDY NECROSIS AND ITS PREVENTION.

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            Orthogonal polarization spectral imaging: a new method for study of the microcirculation.

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              Skin flap failure: pathophysiology.

              C Kerrigan (1983)
              An experimental study was undertaken in pigs to clarify the pathophysiologic events occurring in a failing skin flap. It was demonstrated that skin flaps fail because of arterial insufficiency. Arteriovenous shunting is not a major cause of skin flap necrosis.
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                Author and article information

                Journal
                Clinics (Sao Paulo)
                Clinics (Sao Paulo, Brazil)
                Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
                1807-5932
                1980-5322
                August 2009
                : 64
                : 8
                : 797-802
                Affiliations
                Laboratório de Pesquisas Clínicas e Experimentais em Biologia Vascular, Departamento de Ciências Fisiológicas, Universidade do Estado do Rio de, Janeiro - Rio de Janeiro/RJ, Brazil E-mail: dbottino1@ 123456yahoo.com.br , Tel: 55 21 2587.7771
                Article
                cln64_8p797
                10.1590/S1807-59322009000800015
                2728195
                19690666
                d9581c22-5b5b-4eaf-8efd-f76865eb30ca
                Copyright © 2009 Hospital das Clínicas da FMUSP
                History
                : 24 March 2009
                : 19 May 2009
                Categories
                Clinical Sciences

                Medicine
                ops,microcirculation,skin flap,buflomedilm pentoxifylline
                Medicine
                ops, microcirculation, skin flap, buflomedilm pentoxifylline

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