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      Effects of High Hydrostatic Pressure on the Solubilities and Structures of Alaska Pollock Protein

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          Abstract

          Alaska pollock is an important protein source which is extensively used in the food industry. Pollock protein isolates (PPI) with significantly enriched protein contents could be prepared using isoelectric solubilization/precipitation (ISP) processing; however, the functional properties of this process is limited by the large amount of water-insoluble proteins. In this study, we investigated the influence of high hydrostatic pressure (HHP) treatment on the solubility and structural changes of PPI. PPI obtained using ISP is treated with hydrostatic pressures of 200, 300, 400, and 500 MPa for up to 15 min, and the HHP-treated samples were observed to exhibit significantly improved solubilities. Further biochemical assays reveal that the continuous HHP treatments reduce the contents of free sulfhydryl groups and promote the formation of macromolecules with better water solubilities, which may induce the solubility improvements of the HHP-treated PPI. Our results indicate that HHP can be utilized to effectively prepare highly water-soluble Alaska pollock protein in food processing.

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          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          07 May 2019
          01 April 2019
          : 18
          : 2
          : 413-419
          Affiliations
          1 College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
          2 Zhongshan School of Medicine, SUN YAT-SEN University, Guangzhou 510275, China
          Author notes
          *Corresponding author: LI Bafang
          Article
          s11802-019-3739-3
          10.1007/s11802-019-3739-3
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2019.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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          Self URI (journal-page): https://www.springer.com/journal/11802

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