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      Isolation, Chemical Fingerprinting and Simultaneous Quantification of Four Compounds from Tanacetum gracile Using a Validated HPLC–ESI-QTOF-Mass Spectrometry Method

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          Abstract

          The present study was conducted to carry out the phytochemical investigation of Tanacetum gracile Hook. f. & Thomson and to develop a method for the simultaneous quantification of the isolated compounds in the extracts of T. gracile growing in different locations. Cluster analysis rectangular similarity matrix was performed to understand the chemical fingerprinting variations in the extracts. High-performance liquid chromatography–electrospray ionization-quadrupole-time-of-flight-mass spectrometry (HPLC–ESI-QTOF-MS) was used to quantify four bioactive compounds, and separation of the compounds was achieved on a reverse-phase C8 column using a mobile phase of acetonitrile: 0.1% formic acid in water with a gradient elution by maintaining the flow rate of 300 μL/min. The QTOF–MS was operated using the electro-spray ionization technique with the positive ion polarity mode. The calibration curves of four marker compounds were linear over the concentration range of 3.12–100 ng/µL ( R 2> 0.996). A specific, accurate and precise HPLC–ESI-QTOF-MS method was optimized for the determination of kaempferol, ketoplenolide, tetramethoxyflavone and artemetin both individually and simultaneously. Quantification of these chemical markers in different extracts was carried out using this validated method. Kaempferol was isolated for the first time from T. gracile.

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          Author and article information

          Journal
          J Chromatogr Sci
          J Chromatogr Sci
          chromsci
          chromsci
          Journal of Chromatographic Science
          Oxford University Press
          0021-9665
          1945-239X
          May 2016
          06 March 2016
          : 54
          : 5
          : 796-804
          Affiliations
          [1 ] Analytical Chemistry Division (Instrumentation), CSIR-Indian Institute of Integrative Medicine , Jammu 180001, India
          [2 ] Natural Product Chemistry Division, CSIR-Indian Institute of Integrative Medicine , Jammu 180001, India
          [3 ] Plant Biotechnology Department, CSIR-Indian Institute of Integrative Medicine , Jammu 180001, India
          [4 ] Genetic Resource & Agrotech. Division, CSIR-Indian Institute of Integrative Medicine , Jammu 180001, India
          [5 ] Department of Chemistry, University of Jammu , Jammu 180006, India
          Author notes
          [* ]Author to whom correspondence should be addressed. Email: mkvermadr@ 123456yahoo.com , mkverma@ 123456iiim.ac.in
          Article
          PMC4890456 PMC4890456 4890456 bmw013
          10.1093/chromsci/bmw013
          4890456
          26951542
          © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com
          Categories
          Liquid Chromatography
          Custom metadata
          May/June 2016

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