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      Phylogeny of the Nasonia species complex (Hymenoptera: Pteromalidae) inferred from an internal transcribed spacer (ITS2) and 28S rDNA sequences

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      Insect Molecular Biology
      Wiley-Blackwell

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          Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

          A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus, greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified. In addition, the method was used to amplify and detect a target DNA molecule present only once in a sample of 10(5) cells.
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            Rapid spread of an inherited incompatibility factor in California Drosophila.

            In Drosophila simulans in California, an inherited cytoplasmic incompatibility factor reduces egg hatch when infected males mate with uninfected females. The infection is spreading at a rate of more than 100 km per year; populations in which the infection was rare have become almost completely infected within three years. Analyses of the spread using estimates of selection in the field suggest dispersal distances far higher than those found by direct observation of flies. Hence, occasional long-distance dispersal, possibly coupled with local extinction and recolonization, may be important to the dynamics. Incompatibility factors that can readily spread through natural populations may be useful for population manipulation and important as a post-mating isolating mechanism.
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              Genetics and speciation.

              Called the "mystery of mysteries" by Darwin, speciation is still a little-understood area of evolution. Genetic analysis, however, has yielded new generalizations about speciation and suggests promising avenues of research.
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                Author and article information

                Journal
                Insect Molecular Biology
                Insect Mol Biol
                Wiley-Blackwell
                0962-1075
                1365-2583
                January 1994
                January 1994
                : 2
                : 4
                : 225-237
                Article
                10.1111/j.1365-2583.1994.tb00142.x
                9087560
                d9b49649-2601-4a43-bc47-1be6de70cf0d
                © 1994

                http://doi.wiley.com/10.1002/tdm_license_1.1

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