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      Growth Hormone Stimulates Production of Interferon-Gamma by Human Peripheral Mononuclear Cells

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          Abstract

          There is substantial evidence for interactions between the immune and endocrine systems at different levels. In the present study we investigated whether human growth hormone (hGH) could stimulate proliferation of interferon-γ-secreting cells (IFN-γ-SC), and production of IFN-γ. Human peripheral blood mononuclear cells (PBMC) taken from 15 normal subjects were incubated with varying doses (200,400, 600 and 800 ng/ml) of recombinant hGH. Samples of cells were also incubated with PBS buffer (without hGH) to serve as controls. Effects of hGH were studied by enumerating IFN-γ-SC and by measuring the concentration of IFN-γ using an Immunospot assay and an enzyme-linked immunosorbent assay, respectively. The results showed that hGH significantly increased both the number of IFN-γ-SC and the concentration of IFN-γ in a dose-dependent manner. The maximum effects were obtained in the presence of (400 ng/ml) hGH (15 ± 5 IFN-γ-SC/10<sup>6</sup> PBMC and 300 ± 55 U/ml IFN-γ) compared to controls (4 ± 2 IFN-γ-SC/10<sup>6</sup> PBMC and 50 ± 10 U/ml IFN-γ). The results of the present study suggest that hGH might influence the immune system by stimulating the proliferation of IFN-γ-SC and the production of IFN-γ.

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          Author and article information

          Journal
          HRE
          10.1159/issn.0018-5051
          Hormone Research in Paediatrics
          S. Karger AG
          0018-5051
          2571-6603
          1997
          1997
          10 December 2008
          : 48
          : 1
          : 11-15
          Affiliations
          Department of Clinical Neuroscience and Family Medicine, aGeriatric Section and bSection of Neurology, Karolinska Institute, Stockholm, and cDivision of Biological Research on Drug Dependence, University of Uppsala, Sweden
          Article
          185359 Horm Res 1997;48:11–15
          10.1159/000185359
          © 1997 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 5
          Categories
          Original Paper

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