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      Qualitative detection of ginsenosides in brain tissues after oral administration of high-purity ginseng total saponins by using polyclonal antibody against ginsenosides

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          Abstract

          Given the limited studies and conflicting findings, the transport character of ginsenosides crossing the blood–brain barrier (BBB) remains unclear. The present study was designed to qualitatively determine the distribution of ginsenosides in brain tissues after oral administration of ginseng total saponins, using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) combined with immunohistochemistry. In brain tissue homogenates, ginsenoside Rg1 was detectable and no other ginsenosides or their metabolites were found. No ginsenosides were detected in cerebrospinal fluid. Immunohistochemistry staining of brain tissue sections by using anti-ginsenoside polyclonal antibodies revealed the localization of ginsenosides in brain tissues. Furthermore, immunofluorescence double staining revealed that ginsenosides widely existed in vascular endotheliocytes and astrocytes, and in few neurons. These results indicated that Rg1 was the main component that entered the brain after oral administration of ginseng total saponins and that ginsenosides could cross the BBB, although the transport capability of ginsenosides through the BBB may be poor.

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          Author and article information

          Journal
          CJNM
          Chinese Journal of Natural Medicines
          Elsevier
          1875-5364
          20 March 2018
          : 16
          : 3
          : 175-183
          Affiliations
          1Laboratory of Pathological Sciences, College of Medicine and Life, Nanjing University of Chinese Medicine, Nanjing 210046, China
          2Laboratory of Pathological Sciences, College of Medicine, Yan'an University, Yan’an 716000, China
          3Research Center, Basic Medical College, Nanjing University of Chinese Medicine, Nanjing 210046, China
          Author notes
          *Corresponding author: ZHAO Yu-Nan, Tel/Fax: 86-24-85811922, E-mail: zhaoyunan-js@ 123456163.com

          These authors have no conflict of interest to declare.

          Article
          S1875-5364(18)30045-1
          10.1016/S1875-5364(18)30045-1
          Copyright © 2018 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
          Funding
          Funded by: National Natural Science Foundation of China
          Award ID: 81303246
          Award ID: 81703732
          The work was supported by the National Natural Science Foundation of China (Nos. 81303246, 81703732) and Projects of Priority-Discipline for Colleges and Universities of Jiangsu Province (integration of traditional Chinese and western medicine).

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