The fish sampled in this study were captured in seasonal collections samplings, conducted between May 2006 and February 2007 in 15 stretches of the Corrientes stream basin, Patos lagoon system, Rio Grande do Sul state, Brazil. A total of 8088 specimens were collected, belonging to nine orders, 24 families and 68 species. In general, the ichthyofauna in this basin has the same pattern recognized for Neotropical rivers with predominance of Characiformes and Siluriformes orders and a high representative of the Characidae and Loricariidae families.