Ca2+-Regulated Photoproteins: Effective Immunoassay Reporters

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Abstract

Ca ²⁺-regulated photoproteins of luminous marine coelenterates are of interest and a challenge for researchers as a unique bioluminescent system and as a promising analytical instrument for both in vivo and in vitro applications. The proteins are comprehensively studied as to biochemical properties, tertiary structures, bioluminescence mechanism, etc. This knowledge, along with available recombinant proteins serves the basis for development of unique bioluminescent detection systems that are “self-contained”, triggerable, fast, highly sensitive, and non-hazardous. In the paper, we focus on the use of photoproteins as reporters in binding assays based on immunological recognition element—bioluminescent immunoassay and hybridization immunoassay, their advantages and prospects.

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Author and article information

Journal

Journal ID (nlm-ta): Sensors (Basel)

Title: Sensors (Basel, Switzerland)

Publisher: Molecular Diversity Preservation International (MDPI)

ISSN (Electronic): 1424-8220

Publication date Collection: 2010

Publication date (Electronic): 10 December 2010

Volume: 10

Issue: 12

Pages: 11287-11300

Affiliations

Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 50, Krasnoyarsk, 660036, Russia; E-Mail: lfrank@ 123456yandex.ru ; Tel.: +8-391-249-44-30; Fax: +8-391-243-34-00

Article

Publisher ID: sensors-10-11287

DOI: 10.3390/s101211287

PMC ID: 3231056

PubMed ID: 22163526

SO-VID: da74fed6-1e19-4b2a-9c01-61202280bd4d

License:

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/3.0/).

History

Date received : 29 October 2010

Date revision received : 24 November 2010

Date accepted : 3 December 2010

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