Cell shape controls rheotaxis in small parasitic bacteria

Abstract

Mycoplasmas, a group of small parasitic bacteria, adhere to and move across host cell surfaces. The role of motility across host cell surfaces in pathogenesis remains unclear. Here, we used optical microscopy to visualize rheotactic behavior in three phylogenetically distant species of Mycoplasma using a microfluidic chamber that enabled the application of precisely controlled fluid flow. We show that directional movements against fluid flow occur synchronously with the polarized cell orienting itself to be parallel against the direction of flow. Analysis of depolarized cells revealed that morphology itself functions as a sensor to recognize rheological properties that mimic those found on host-cell surfaces. These results demonstrate the vital role of cell morphology and motility in responding to mechanical forces encountered in the native environment.

Author summary

The small, parasitic bacterium Mycoplasma pneumoniae attaches to, and moves over, host cell surfaces. Adherence to host surfaces and motility are critical for the pathogenicity of M. pneumoniae. The role of motility by M. pneumoniae in vivo, however, is poorly understood. Host airways generate constant fluid flow toward the mouth as part of their defense against pathogens and irritants. Consequently, pulmonary invaders must counter the rheological forces found in host airways in order to successfully colonize the host. Here, we demonstrate that M. pneumoniae exhibits directional movement against fluid flow. These findings suggest there is a vital role for rheotactic motility that has evolved in order to overcome host defense mechanisms such as mucociliary clearance.