28
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Cytotoxic and apoptotic potential of Myristica fragrans Houtt. (mace) extract on human oral epidermal carcinoma KB cell lines

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Several studies have revealed that certain naturally occurring medicinal plants inhibit the growth of various cancers. The present study was conducted to evaluate cytotoxicity and apoptotic induction potential of Myristica fragrans Houtt mace extract. The cytotoxic activity of the Myristica fragrans Houtt mace acetone extract was assayed by MTT assay on human oral epidermal carcinoma KB cell lines. KB cells were incubated with different concentration of mace extract ranging from 25 to 125 μg/mL for 24hrs. The apoptotic induction potential was also studied by the analysis of Bcl-2 protein and gene expression in mace extract incubated KB cell lines using western blotting technique and real-time polymerase chain reaction. The mace extract exhibited cytotoxicity and anticancer effect against KB cell lines and it also suppressed the growth of cancer cells, therefore growth inhibitory effect was noted in extract treated cell lines. The apoptotic potential of mace extract was accompanied by reduced gene expression of Bcl-2 compared to the untreated KB cells. The mace extract shows the cytotoxic activity and induced the apoptosis through the modulation of its target genes Bcl-2 in the KB cell lines, suggesting the potential of mace as a candidate for oral cancer chemoprevention. This can be further investigated in vivo for its anticancer potential.

          Related collections

          Most cited references21

          • Record: found
          • Abstract: not found
          • Article: not found

          Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays

            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Myristica fragrans Suppresses Tumor Growth and Metabolism by Inhibiting Lactate Dehydrogenase A.

            Most cancer cells predominantly produce ATP by maintaining a high rate of lactate fermentation, rather than by maintaining a comparatively low rate of tricarboxylic acid cycle, i.e., Warburg's effect. In the pathway, the pyruvate produced by glycolysis is converted to lactic acid by lactate dehydrogenase (LDH). Here, we demonstrated that water extracts from the seeds of Myristica fragrans Houtt. (MF) inhibit the in vitro enzymatic activity of LDH. MF effectively suppressed cell growth and the overall Warburg effect in HT29 human colon cancer cells. Although the expression of LDH-A was not changed by MF, both lactate production and LDH activity were decreased in MF-treated cells under both normoxic and hypoxic conditions. In addition, intracellular ATP levels were also decreased by MF treatment, and the uptake of glucose was also reduced by MF treatment. Furthermore, the experiment on tumor growth in the in vivo mice model revealed that MF effectively reduced the growth of allotransplanted Lewis lung carcinoma cells. Taken together, these results suggest that MF effectively inhibits cancer growth and metabolism by inhibiting the activity of LDH, a major enzyme responsible for regulating cancer metabolism. These results implicate MF as a potential candidate for development into a novel drug against cancer through inhibition of LDH activity.
              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Global cancer statistics

                Bookmark

                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                bjps
                Brazilian Journal of Pharmaceutical Sciences
                Braz. J. Pharm. Sci.
                Universidade de São Paulo, Faculdade de Ciências Farmacêuticas (São Paulo, SP, Brazil )
                2175-9790
                November 2018
                : 54
                : 3
                : e18028
                Affiliations
                [1] Coimbatore Tamil Nadu orgnameBharathiar University orgdiv1Department Of Biochemistry orgdiv2Research and Development Centre India
                [3] Chennai Tamil Nadu orgnameSaveetha University orgdiv1Department of Biochemistry orgdiv2Saveetha Dental College and Hospital India
                [4] Chennai West Bengal orgnameIndian Institute Of Science Education And Research orgdiv1Department of Biochemistry India
                [2] Chennai West Bengal orgnameIndian Institute Of Science Education And Research orgdiv1Department of Biochemistry India
                Article
                S1984-82502018000300629
                10.1590/s2175-97902018000318028
                db1a2cde-c6c3-4491-942a-e68454419b83

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 14 January 2018
                : 23 January 2018
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 21, Pages: 0
                Product

                SciELO Brazil

                Categories
                Article

                Cytotoxicity,MTT assay,(mace)/extract,Myristica fragrans Houtt,KB cell lines,Apoptosis,Bcl-2 gene

                Comments

                Comment on this article