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Fluorogenic probe for fast 3D whole-cell DNA-PAINT
Preprint
Kenny Kwok Hin Chung ,
Zhao Zhang ,
Phylicia Kidd ,
Yongdeng Zhang ,
Nathan D Williams ,
Bennett Rollins ,
Yang Yang ,
Chenxiang Lin ,
David Baddeley ,
Joerg Bewersdorf
April 30 2020
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Abstract
DNA-PAINT is an increasingly popular super-resolution microscopy method that can acquire high-fidelity images at nanometer resolution. It suffers, however, from high background and very slow imaging speed, both of which can be attributed to the presence of unbound fluorophores in solution. We present a fluorogenic DNA-PAINT probe that solves these problems and demonstrate 3D imaging without the need for optical sectioning and a 26-fold increase in imaging speed over regular DNA-PAINT.