1
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      MicroRNA-665-3p attenuates oxygen-glucose deprivation-evoked microglial cell apoptosis and inflammatory response by inhibiting NF-κB signaling via targeting TRIM8.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Microglial inflammation induced by ischemic stroke aggravates brain damage. MicroRNAs (miRNAs) have emerged as pivotal regulators in ischemic stroke-induced inflammation in microglial cells. miR-665-3p has been reported as a critical inflammation-associated miRNA. However, whether miR-665-3p participates in regulating microglial inflammation during ischemic stroke is underdetermined. This study investigated the potential role of miR-665-3p in stroke-induced inflammation in microglial cells using a cellular model of oxygen-glucose deprivation (OGD)-stimulated microglial cells in vitro. We found that miR-665-3p expression was decreased in microglial cells exposed to OGD treatment. Functional experiments demonstrated that the overexpression of miR-665-3p attenuated OGD-induced apoptosis and inflammation in microglial cells. Notably, tripartite motif 8 (TRIM8) was identified as a target gene of miR-665-3p. TRIM8 expression was induced by OGD treatment in microglial cells and the knockdown of TRIM8 protected microglial cells from OGD -induced cytotoxicity and inflammation. Moreover, TRIM8 knockdown or miR-665-3p overexpression blocked OGD-induced activation of nuclear factor (NF)-κB signaling in microglial cells. In addition, TRIM8 overexpression partially reversed the miR-665-3p overexpression-mediated inhibitory effect on OGD-induced inflammation in microglial cells. Taken together, these results indicate that miR-665-3p up-regulation protects microglial cells from OGD-induced apoptosis and inflammatory response by targeting TRIM8 to inhibit NF-κB signaling.

          Related collections

          Author and article information

          Journal
          Int Immunopharmacol
          International immunopharmacology
          Elsevier BV
          1878-1705
          1567-5769
          Aug 2020
          : 85
          Affiliations
          [1 ] Department of Anesthesiology, Xi'an Fourth Hospital, Xi'an 710004, China.
          [2 ] Department of Anesthesiology, Second Affiliated Hospital of Air Force Medical University, Xi'an 710038, China.
          [3 ] Department of Anesthesiology, Second Affiliated Hospital of Air Force Medical University, Xi'an 710038, China. Electronic address: sunxude@fmmu.edu.com.
          Article
          S1567-5769(20)30618-4
          10.1016/j.intimp.2020.106650
          32512270
          db53f105-1571-4328-8200-909262d65383

          Inflammation, TRIM8, NF-κB, miR-665-3p, Microglia

          Comments

          Comment on this article