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      Mutual binding inhibition of tetrodotoxin and saxitoxin to their binding protein from the plasma of the puffer fish, Fugu pardalis.

      Bioscience, biotechnology, and biochemistry

      blood, Animals, Binding, Competitive, Blood Proteins, metabolism, Chromatography, High Pressure Liquid, Inhibitory Concentration 50, Protein Binding, Saxitoxin, antagonists & inhibitors, Tetraodontiformes, Tetrodotoxin

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          Abstract

          The mutual binding inhibition of tetrodotoxin and saxitoxin to their binding protein from the plasma of Fugu pardalis was investigated by HPLC. The values for the half inhibitory concentration of tetrodotoxin (1.6 microM) binding to this protein (1.2 microM) for saxitoxin, and of saxitoxin (0.47 microM) binding to that (0.30 microM) for tetrodotoxin were 0.35 +/- 0.057 microM and 81 +/- 16 microM (n = 2), respectively.

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          12507001

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