17
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      A simple turn on fluorescent sensor for the selective detection of thiamine using coconut water derived luminescent carbon dots.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          In this study microwave-assisted hydrothermal method was used to prepare highly luminescent carbon dots (1-6 nm size) within a minute from tender coconut (Cocos nucifera) water. The synthesized carbon dots (C-dots) exhibit emission of blue and green lights while excited at 390 and 450 nm wavelengths, respectively. As an application, these C-dots were tested for a simple "turn on" fluorescent sensor for rapid detection of thiamine (vitamin B1). The detection of thiamine in human body is very important to prevent various diseases such as beriberi, neurological disorders, optic neuropathy, etc. The fluorescence emission intensity of C-dots quenches after addition of Cu(2+) ion and then again increases selectively (turn on) after the addition of thiamine. The fluorescence emission intensity enhancement of Cu(2+) ion modified C-dots in the presence of thiamine exhibits a linear relationship within the thiamine concentration range of 10-50 μM. The limit of detection was found to be 280 nM from this study. The selectivity of the detection was also tested in the presence of different organic molecules and inorganic ions (Ca(2+), Mg(2+), Na(+), K(+), Cl(-), SO4(2-), and NO3(-)) which are present in blood serum and urine and found to be almost no interference in the detection. Finally, to see the applicability in real samples a commercial vitamin capsule was tested and found less than 3% error in the detected concentration. The C-dots were also used for bioimaging of fungus and the results show they are also suitable for this application too.

          Related collections

          Author and article information

          Journal
          Biosens Bioelectron
          Biosensors & bioelectronics
          Elsevier BV
          1873-4235
          0956-5663
          May 15 2016
          : 79
          Affiliations
          [1 ] Interfaces and Nanomaterials Laboratory, Department of Chemical Engineering, National Institute of Technology, Rourkela 769008, India.
          [2 ] Interfaces and Nanomaterials Laboratory, Department of Chemical Engineering, National Institute of Technology, Rourkela 769008, India. Electronic address: sparia@nitrkl.ac.in.
          Article
          S0956-5663(15)30740-5
          10.1016/j.bios.2015.12.087
          26745793
          dc172e89-6bd8-4910-9a67-f451fd760290
          History

          Bioimaging,Carbon dot,Microwave-hydrothermal,Photoluminescence,Tender coconut water,Thiamine sensor

          Comments

          Comment on this article