In vivo Brillouin optical microscopy of the human eye

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

We report the first Brillouin measurement of the human eye in vivo. We constructed a Brillouin optical scanner safe for human use by employing continuous-wave laser light at 780 nm at a low power of 0.7 mW. With a single scan along the optic axis of the eye, the axial profile of Brillouin frequency shift was obtained with a pixel acquisition time of 0.4 s and axial resolution of about 60 μm, showing the depth-dependent biomechanical properties in the cornea and lens.

Related collections

Most cited references 25

Record: found
Abstract: found
Article: not found

Determining in vivo biomechanical properties of the cornea with an ocular response analyzer.

David Luce (2005)

To study the results of an ocular response analyzer (ORA) to determine the biomechanical properties of the cornea and their relationship to intraocular pressure (IOP). Reichert Inc., Depew, New York, USA. The ORA (Reichert) makes 2 essentially instantaneous applanation measurements that permit determination of corneal and IOP effects. Measurements of several populations indicate that corneal hysteresis, a biomechanical measure, varied over a dynamic range of 1.8 to 14.6 mm Hg and was only weakly correlated with corneal thickness (r(2)=0.12); this is related to the observation that some subjects with relatively thick corneas have less-than-average corneal hysteresis. Corneal hysteresis changes diurnally, presumably as a result of hydration changes. Keratoconus, Fuchs' dystrophy, and post-LASIK patients demonstrated low corneal hysteresis. The corneal hysteresis biomechanical measure may prove valuable for qualification and predictions of outcomes of refractive surgery and in other cases in which corneal biomechanics are important.

0 comments Cited 292 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Confocal Brillouin microscopy for three-dimensional mechanical imaging.

Giuliano Scarcelli, Seok Hyun Yun (2007)

Acoustically induced inelastic light scattering, first reported in 1922 by Brillouin1, allows non-contact, direct readout of the viscoelastic properties of a material and has widely been investigated for material characterization2, structural monitoring3 and environmental sensing4. Extending the Brillouin technique from point sampling spectroscopy to imaging modality5 would open up new possibilities for mechanical imaging, but has been challenging because rapid spectrum acquisition is required. Here, we demonstrate a confocal Brillouin microscope based on a fully parallel spectrometer-a virtually imaged phased array-that improves the detection efficiency by nearly 100-fold over previous approaches. Using the system, we show the first cross-sectional Brillouin imaging based on elastic properties as the contrast mechanism and monitor fast dynamic changes in elastic modulus during polymer crosslinking. Furthermore, we report the first in situ biomechanical measurement of the crystalline lens in a mouse eye. These results suggest multiple applications of Brillouin microscopy in biomedical and biomaterial science.

0 comments Cited 132 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: not found
Article: not found

Maximum permissible exposures for ocular safety (ANSI 2000), with emphasis on ophthalmic devices

François C Delori, Robert H Webb, David Sliney (2007)

0 comments Cited 115 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Journal

Journal ID (nlm-ta): Opt Express

Journal ID (iso-abbrev): Opt Express

Journal ID (publisher-id): OE

Title: Optics Express

Publisher: Optical Society of America

ISSN (Electronic): 1094-4087

Publication date (Electronic): 05 April 2012

Publication date Collection: 09 April 2012

Publication date PMC-release: 05 April 2013

Volume: 20

Issue: 8

Pages: 9197-9202

Affiliations

[1]Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 50 Blossom St., BHX-6, Boston, Massachusetts 02114, USA

Author notes

[* ] syun@ 123456hms.harvard.edu

Article

Publisher ID: 163291

DOI: 10.1364/OE.20.009197

PMC ID: 3500092

PubMed ID: 22513631

SO-VID: dc19448a-e423-41c1-93b5-730ada57828b

License:

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.