Ionic Conductances in Cultured Pre-Infundibular Cells from the Hypothalamic Arcuate Region

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Abstract

The hypothalamic arcuate nucleus plays an important role in the gating system controlling the secretion of hypothalamic neurons. In order to analyze this gating mechanism, arcuate neurons from rats aged 21–22 days were cultured in a chemically defined medium. Addition of nerve growth factor to this medium increased the survival of the arcuate neurons. Neuron characterization was done with the Lucifer Yellow liposome technique and neurofilament immunocytochemistry. Electrophysiological information was obtained with the patch-clamp technique by whole-cell recordings and single-channel measurements. This qualitative inventory demonstrated the presence of at least five types of conductances: a sodium conductance, two potassium conductances, a calcium-activated conductance, presumably determined by potassium, and a leakage conductance.

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Journal

Journal ID (publisher-id): NEN

Journal ID (nlm-ta): Neuroendocrinology

Journal ID (doi): 10.1159/issn.0028-3835

Title: Neuroendocrinology

Publisher: S. Karger AG

ISSN (Print): 0028-3835

ISSN (Electronic): 1423-0194

Publication date Subscription-year: 1988

Publication date (Print): 1988

Publication date (Electronic): 02 April 2008

Volume: 48

Issue: 4

Pages: 445-452

Affiliations

^aLaboratory of Anatomy and Embryology and ^bDepartment of Physiology, University of Leiden, and ^cLeiden Cytologic Laboratory, Leiden, The Netherlands

Article

Publisher ID: 125047 Other ID: Neuroendocrinology 1988;48:445–452

DOI: 10.1159/000125047

PubMed ID: 2463495

SO-VID: dc26c7ff-4e50-470e-a60d-669a225d8dc5

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Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 29 June 1987

Date accepted : 03 March 1988

Page count

Pages: 8

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