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      Ionic Conductances in Cultured Pre-Infundibular Cells from the Hypothalamic Arcuate Region

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          Abstract

          The hypothalamic arcuate nucleus plays an important role in the gating system controlling the secretion of hypothalamic neurons. In order to analyze this gating mechanism, arcuate neurons from rats aged 21–22 days were cultured in a chemically defined medium. Addition of nerve growth factor to this medium increased the survival of the arcuate neurons. Neuron characterization was done with the Lucifer Yellow liposome technique and neurofilament immunocytochemistry. Electrophysiological information was obtained with the patch-clamp technique by whole-cell recordings and single-channel measurements. This qualitative inventory demonstrated the presence of at least five types of conductances: a sodium conductance, two potassium conductances, a calcium-activated conductance, presumably determined by potassium, and a leakage conductance.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1988
          1988
          02 April 2008
          : 48
          : 4
          : 445-452
          Affiliations
          aLaboratory of Anatomy and Embryology and bDepartment of Physiology, University of Leiden, and cLeiden Cytologic Laboratory, Leiden, The Netherlands
          Article
          125047 Neuroendocrinology 1988;48:445–452
          10.1159/000125047
          2463495
          © 1988 S. Karger AG, Basel

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          Page count
          Pages: 8
          Categories
          Original Paper

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