7
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      The AtGRXS3/4/5/7/8 glutaredoxin gene cluster on Arabidopsis thaliana chromosome 4 is coordinately regulated by nitrate and appears to control primary root growth

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          ABSTRACT

          Nitrate and ammonium are the 2 most common forms of inorganic nitrogen available to plants in the soil. We previously identified a group of class III glutaredoxin genes whose expression is strongly upregulated by nitrate, but not ammonium, in Arabidopsis thaliana shoots and roots. A reverse genetics approach was used to functionally characterize a subset of these nitrate-regulated glutaredoxins, and we found that the AtGRXS3,4,5, and 8 genes function as negative regulators of primary root growth. AtGRXS3/4/5/8 are arranged in a tandem array on Arabidopsis chromosome 4, and these genes show very high levels of sequence similarity. Interestingly, there is one additional glutaredoxin, AtGRXS7, in this same gene cluster, but this gene was not identified as nitrate-responsive in our previous studies. We show here that AtGRXS7 is upregulated by nitrate and shows strong co-expression with the other glutaredoxins in this gene cluster. Further, AtGRXS7 was effectively silenced by the RNAi construct used to target AtGRXS3/4/5/8 for previous functional analyses. Overall, it appears that the 5 genes in the AtGRX3/4/5/7/8 cluster share virtually identical sequences, regulatory patterns, and functions, collectively acting to regulate primary root growth in response to soil nitrate.

          Related collections

          Author and article information

          Journal
          Plant Signal Behav
          Plant Signal Behav
          KPSB
          Plant Signaling & Behavior
          Taylor & Francis
          1559-2316
          1559-2324
          April 2016
          6 April 2016
          : 11
          : 4
          : e1171450
          Affiliations
          Department of Biological Sciences, California State University San Marcos , San Marcos, California, USA
          Author notes
          CONTACT Matthew A. Escobar mescobar@ 123456csusm.edu
          Article
          PMC4883855 PMC4883855 4883855 1171450
          10.1080/15592324.2016.1171450
          4883855
          27049601
          dc5b0dcb-41a7-4f66-a28a-2b78a7916b87
          © 2016 Taylor & Francis Group, LLC
          History
          : 2 March 2016
          : 18 March 2016
          : 21 March 2016
          Page count
          Figures: 2, Tables: 1, References: 6, Pages: 3
          Categories
          Short Communication

          plant development,nitrate,glutaredoxins, AtGRXS7 , Arabidopsis thaliana ,primary root growth

          Comments

          Comment on this article