Erratum : Treatment of Dystrophic  mdx  Mice with an ADAMTS-5 Specific Monoclonal Antibody Increases the Ex Vivo Strength of Isolated Fast Twitch Hindlimb Muscles.  Biomolecules  2020,  10 , 416

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

The authors wish to make a change to this published paper [1]. In the original manuscript, there was a mistake in the labeling of the y-axis for Figure 5B,D. To assess muscle fatiguability, extensor digitorum longus (EDL) and soleus muscles were subjected to 4 min of intermittent (1 contraction every 5 s), submaximal stimulation at 60 Hz. The force output of every fifth contraction is statistically presented as a data point. The revision to Figure 5B,D now reflects the correct stimulation number. The authors apologize for any inconvenience caused, and we wish to stress that this change does not affect the presented scientific results and statistical analyses. The manuscript with be updated and the original will remain online on the article webpage at https://www.mdpi.com/2218-273X/10/3/416.

Related collections

Most cited references 1

Record: found
Abstract: found
Article: found

Is Open Access

Treatment of Dystrophic mdx Mice with an ADAMTS-5 Specific Monoclonal Antibody Increases the Ex Vivo Strength of Isolated Fast Twitch Hindlimb Muscles

Alex B. Addinsall, Leonard Forgan, Natasha L. McRae … (2020)

Aberrant extracellular matrix synthesis and remodeling contributes to muscle degeneration and weakness in Duchenne muscular dystrophy (DMD). ADAMTS-5, a secreted metalloproteinase with catalytic activity against versican, is implicated in myogenesis and inflammation. Here, using the mdx mouse model of DMD, we report increased ADAMTS-5 expression in dystrophic hindlimb muscles, localized to regions of regeneration and inflammation. To investigate the pathophysiological significance of this, 4-week-old mdx mice were treated with an ADAMTS-5 monoclonal antibody (mAb) or IgG2c (IgG) isotype control for 3 weeks. ADAMTS-5 mAb treatment did not reduce versican processing, as protein levels of the cleaved versikine fragment did not differ between hindlimb muscles from ADAMTS-5 mAb or IgG treated mdx mice. Nonetheless, ADAMTS-5 blockade improved ex vivo strength of isolated fast extensor digitorum longus, but not slow soleus, muscles. The underpinning mechanism may include modulation of regenerative myogenesis, as ADAMTS-5 blockade reduced the number of recently repaired desmin positive myofibers without affecting the number of desmin positive muscle progenitor cells. Treatment with the ADAMTS-5 mAb did not significantly affect markers of muscle damage, inflammation, nor fiber size. Altogether, the positive effects of ADAMTS-5 blockade in dystrophic muscles are fiber-type-specific and independent of versican processing.

0 comments Cited 6 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Journal

Journal ID (nlm-ta): Biomolecules

Journal ID (iso-abbrev): Biomolecules

Journal ID (publisher-id): biomolecules

Title: Biomolecules

Publisher: MDPI

ISSN (Electronic): 2218-273X

Publication date (Electronic): 15 July 2020

Publication date Collection: July 2020

Publication date PMC-release: 15 July 2020

Volume: 10

Issue: 7

Electronic Location Identifier: 1053

Affiliations

[1 ]Centre for Molecular and Medical Research, School of Medicine, Deakin University, Waurn Ponds, VIC 3216, Australia; alex.addinsall@ 123456ki.se (A.B.A.); leonard.forgan@ 123456deakin.edu.au (L.G.F.); natasha.mcrae1@ 123456deakin.edu.au (N.L.M.); rwkelly@ 123456deakin.edu.au (R.W.K.); penny.mcdonald@ 123456mcri.edu.au (P.L.M.); bryony.mcneill@ 123456deakin.edu.au (B.M.); daniel.mcculloch@ 123456uq.net.au (D.R.M.)

[2 ]Department of Physiology and Pharmacology, Karolinska Insitutet, 171 77 Stockholm, Sweden

[3 ]Department of Medicine—Western Health, The University of Melbourne, St. Albans, VIC 3021, Australia

[4 ]Australian Institute for Musculoskeletal Science (AIMSS), 176 Furlong Road, St. Albans, VIC 3021, Australia

Author notes

[* ]Correspondence: nicole.stupka@ 123456unimelb.edu.au ; Tel.: +613-8395-8228

[†]

Authors contributed equally.

Article

Publisher ID: biomolecules-10-01053

DOI: 10.3390/biom10071053

PMC ID: 7408084

PubMed ID: 32679807

SO-VID: dc9c798b-0ea0-4f01-8a89-b780c53334bc

License:

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

History

Date received : 09 July 2020

Date accepted : 10 July 2020

Comments

Comment on this article

scite_

Cited by 1

Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Authors: Prech Uapinyoying, Marshall Hogarth, Surajit Battacharya …

See all cited by

Most referenced authors 8

See all reference authors

Erratum: Treatment of Dystrophic mdx Mice with an ADAMTS-5 Specific Monoclonal Antibody Increases the Ex Vivo Strength of Isolated Fast Twitch Hindlimb Muscles. Biomolecules 2020, 10, 416

Read this article at