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      Age-related expression of TGF beta family receptors in human cumulus oophorus cells

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          Abstract

          Purpose

          During ovarian follicle growth, local cellular interactions are essential for oocyte quality acquisition and successful fertilization. While cumulus cells (CCs) nurture oocytes, they also deliver oocyte-secreted factors (OSFs) that activate receptors on CCs. We hypothesized that disturbance of those interactions contributes to age-related lower reproductive success in women submitted to assisted reproductive technology treatments.

          Methods

          Women aged 27–48, without recognized personal reproductive disorder, were enrolled in the study and divided in <35- and ≥35-year-old groups. CCs collected upon follicle aspiration were processed for immunocytochemistry and RNA extraction. The expression patterns of OSF receptors BMPR2, ALK 4, ALK5, and activin receptor-like kinase (ALK6) were studied.

          Results

          Independently of age, receptors were found mostly in the cell periphery. The quantitative assay revealed that in older women, BMPR2, ALK 4, and ALK6 were all significantly decreased, whereas ALK5 was slightly increased.

          Conclusions

          Female age imparts an effect on the expression of OSF receptors in CCs. The findings indicate that reproductive aging affects the local regulation of signaling pathways mediated by BMPR2, ALK6, and ALK4 receptor activation, suggesting their joint involvement.

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          Author and article information

          Contributors
          +351 937 323 980 , angela.ribeiro@ceti.pt
          Journal
          J Assist Reprod Genet
          J. Assist. Reprod. Genet
          Journal of Assisted Reproduction and Genetics
          Springer US (New York )
          1058-0468
          1573-7330
          2 May 2017
          September 2017
          : 34
          : 9
          : 1121-1129
          Affiliations
          [1 ]Centro de Estudo e Tratamento da Infertilidade (CETI), 4100-118 Porto, Portugal
          [2 ] ISNI 0000 0001 1503 7226, GRID grid.5808.5, IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, , Instituto de Investigação e Inovação em Saúde - i3S, Universidade do Porto, ; 4200-135 Porto, Portugal
          [3 ] ISNI 0000 0001 1503 7226, GRID grid.5808.5, Biomedicine Department, Faculty of Medicine, , University of Porto, ; 4200-319 Porto, Portugal
          [4 ] ISNI 0000 0001 1503 7226, GRID grid.5808.5, Reprodutive Medicine, Obstetrics and Gynecology, , Hospital Dr. Nélio Mendonça, SESARAM, 9004-541 Funchal, Portugal; Obstetrics-Gynecology Department, University of Porto, ; Porto, Portugal
          [5 ] ISNI 0000 0001 1503 7226, GRID grid.5808.5, Faculty of Nutrition and Food Sciences, , University of Porto, ; 4200-465 Porto, Portugal
          [6 ] ISNI 0000 0001 1503 7226, GRID grid.5808.5, Obstetrics-Gynecology Department, , University of Porto, ; 4200-319 Porto, Portugal
          [7 ]Obstetrics-Gynecology, Hospital CUF Porto, 4100-180 Porto, Portugal
          Author information
          http://orcid.org/0000-0003-1453-5631
          Article
          PMC5581779 PMC5581779 5581779 930
          10.1007/s10815-017-0930-6
          5581779
          28466233
          dce05aff-983f-4150-ab99-f38c370a778d
          © Springer Science+Business Media New York 2017
          History
          : 25 October 2016
          : 19 April 2017
          Funding
          Funded by: Merck KGaA (DE)
          Award ID: Grant for Fertility Innovation
          Categories
          Reproductive Physiology and Disease
          Custom metadata
          © Springer Science+Business Media, LLC 2017

          Aging,Cumulus cells,Female infertility,Gene expression,Growth factors

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