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Identification and characterization of a novel mammalian isoform of the endocytic adaptor ITSN1
Author(s):
Mykola Dergai
,
Inessa Skrypkina
,
Oleksandr Dergai
,
Liudmyla Tsyba
,
Olga Novokhatska
,
Valeriy Filonenko
,
Lyudmyla Drobot
,
Alla Rynditch
Publication date
Created:
October 2011
Publication date
(Print):
October 2011
Journal:
Gene
Publisher:
Elsevier BV
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Abstract
Intersectin 1 (ITSN1) is an evolutionarily conserved adaptor protein engaged in clathrin-mediated endocytosis, cell signaling and actin cytoskeleton rearrangements. Two major ITSN1 isoforms were initially described, the ubiquitous short isoform (ITSN1-s) and the long isoform (ITSN1-l) expressed predominantly in neurons. Numerous alternative splicing events for ITSN1 pre-mRNA were later identified. Here we describe a novel isoform ITSN1-22a with an alternative C-terminus encoded by exon 22a. This exon is only found in placental mammals. The transcript of ITSN1-22a is detected in a wide range of human and mouse tissues. We show here that two alternative splicing events affect the coding sequence of the ITSN1-22a isoform. Moreover, alternative polyadenylation of these transcripts was demonstrated in human tissues. The protein encoded by the ITSN1-22a transcript possesses two EH domains, a coiled-coil region, an SH3A domain and a specific C-terminal domain (CTD) but lacks four SH3 domains in comparison with ITSN1-s. The level of ITSN1-22a protein varies in different mouse tissues and human cell lines. The highest amounts of this isoform occur in mouse brain, spleen, lung and the human B cell line DG75. ITSN1-22a binds via its CTD to the SH3 domain of the endocytic protein amphiphysin 1 and the SH3A domain of ITSN1. Furthermore association in vivo and codistribution of ITSN1-22a and ITSN1-s were demonstrated suggesting that these isoforms could function in concert. We have revealed differential binding of ITSN1-s and ITSN1-22a to the ubiquitin ligase Cbl. Both isoforms possess the SH3A domain capable of binding to Cbl; however ITSN1-22a in contrast to ITSN1-s did not interact with Cbl in vivo. In vitro binding experiments demonstrated that the CTD of ITSN1-22a negatively regulated its binding to Cbl; at the same time interaction with another partner, dynamin 1 was not affected by the presence of the CTD. These data suggest that intramolecular interaction within ITSN1-22a could specifically regulate its binding to protein partners. Thus, this novel mammalian ITSN1 isoform possesses a significantly altered domain structure and performs specific protein-protein interactions. Copyright © 2011 Elsevier B.V. All rights reserved.
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Journal
Title:
Gene
Abbreviated Title:
Gene
Publisher:
Elsevier BV
ISSN (Print):
03781119
Publication date Created:
October 2011
Publication date (Print):
October 2011
Volume
: 485
Issue
: 2
Pages
: 120-129
Article
DOI:
10.1016/j.gene.2011.06.021
PubMed ID:
21712076
SO-VID:
dd457614-2b31-4960-9502-4aced2c0177d
Copyright ©
© 2011
License:
https://www.elsevier.com/tdm/userlicense/1.0/
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