Blog
About

  • Record: found
  • Abstract: found
  • Article: not found

Human complement component factor B rescues HIV-1-infected leukemic T cells from cytopathic death.

Read this article at

ScienceOpenPubMed
Bookmark
      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

      Abstract

      The addition of 5-10% of fresh normal human serum (NHS) from normal individuals into a culture of primarily HIV-1-infected CD4+ leukemic T-cell lines CEM and MT4 was found to rescue the infected cells from cytopathic death, enabling the cells to achieve growth within 10 days. The HIV-1-infected cells cultured in ordinary medium with fetal calf serum (FCS) all died within 10 days. The effect of NHS was ascribed to human complement component factor B and one or more factor B-dependent heat-labile co-factors. The cells which survived in the presence of NHS rapidly lost surface expression of CD4 and became completely resistant to rechallenge by HIV-1. Viral genomes were dramatically reduced in surviving cells within 30 days, and one cell-line CEM completely expelled them during this period. The results suggest that factor B has protective and potential therapeutic significance in HIV-1 infection.

      Related collections

      Author and article information

      Affiliations
      [1 ] Department of Microbiology, Tokyo Medical College, Japan.
      Journal
      Int. Immunol.
      International immunology
      0953-8178
      0953-8178
      Apr 1991
      : 3
      : 4
      1878341

      Comments

      Comment on this article