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One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly
Author(s):
Sergey Koren
,
Adam M Phillippy
Publication date
Created:
February 2015
Publication date
(Print):
February 2015
Journal:
Current Opinion in Microbiology
Publisher:
Elsevier BV
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Abstract
Like a jigsaw puzzle with large pieces, a genome sequenced with long reads is easier to assemble. However, recent sequencing technologies have favored lowering per-base cost at the expense of read length. This has dramatically reduced sequencing cost, but resulted in fragmented assemblies, which negatively affect downstream analyses and hinder the creation of finished (gapless, high-quality) genomes. In contrast, emerging long-read sequencing technologies can now produce reads tens of kilobases in length, enabling the automated finishing of microbial genomes for under $1000. This promises to improve the quality of reference databases and facilitate new studies of chromosomal structure and variation. We present an overview of these new technologies and the methods used to assemble long reads into complete genomes. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.
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Microbial Genomics
Author and article information
Journal
Title:
Current Opinion in Microbiology
Abbreviated Title:
Current Opinion in Microbiology
Publisher:
Elsevier BV
ISSN (Print):
13695274
Publication date Created:
February 2015
Publication date (Print):
February 2015
Volume
: 23
Pages
: 110-120
Article
DOI:
10.1016/j.mib.2014.11.014
PubMed ID:
25461581
SO-VID:
dd8f1b4e-0332-452e-bf96-576c7b786854
Copyright ©
© 2015
License:
https://www.elsevier.com/tdm/userlicense/1.0/
http://creativecommons.org/licenses/by/3.0/
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