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      One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly

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      Current Opinion in Microbiology
      Elsevier BV

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          Abstract

          Like a jigsaw puzzle with large pieces, a genome sequenced with long reads is easier to assemble. However, recent sequencing technologies have favored lowering per-base cost at the expense of read length. This has dramatically reduced sequencing cost, but resulted in fragmented assemblies, which negatively affect downstream analyses and hinder the creation of finished (gapless, high-quality) genomes. In contrast, emerging long-read sequencing technologies can now produce reads tens of kilobases in length, enabling the automated finishing of microbial genomes for under $1000. This promises to improve the quality of reference databases and facilitate new studies of chromosomal structure and variation. We present an overview of these new technologies and the methods used to assemble long reads into complete genomes. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

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          Author and article information

          Journal
          Current Opinion in Microbiology
          Current Opinion in Microbiology
          Elsevier BV
          13695274
          February 2015
          February 2015
          : 23
          : 110-120
          Article
          10.1016/j.mib.2014.11.014
          25461581
          dd8f1b4e-0332-452e-bf96-576c7b786854
          © 2015

          https://www.elsevier.com/tdm/userlicense/1.0/

          http://creativecommons.org/licenses/by/3.0/

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