Objective To discuss and explore the distribution characteristics of intestinal flora in children with attention deficit hyperactivity disorder (ADHD) complicated with obesity.
Methods 64 children with attention deficit hyperactivity disorder (ADHD) admitted to Hangzhou Seventh people’s Hospital from August 2018 to October 2019 were divided into 32 children with normal weight ADHD (control group) and 32 obese children with ADHD (experimental observation group). The macrogenomic sequencing method of faecal flora was used to compare the a diversity of microflora, relative abundance of species classification and differences in thenumber of bacteria between the two groups of children.
Results No significant difference in the α diversity of intestinal microflora was observed between the experimental group and control group (all P>0.05). With regard to bacteria genus, the abundance of Veillonella and Enterococcus in the experimental observation group was lower than that in the control group, but the abundance of Flavobacteriaceae and Enterococcus in the experimental group was higher than that in the control group (all P<0.05). With regard to bacteria species, the abundance of Lachnospira, Prausnitzii, Veillonella and active rumen cocci in the experimental group was lower than that in the control group, whereas the abundance of Pseudomonas aeruginosa, Bacteroides faecalii and Bacteroides viscera in the experimental group was higher than that in the control group (all P<0.05). The number of rectal true bacteria in the control group was significantly higher than that in the experimental group, whereas the number of Bacteroides polymorpha, Bifidobacterium and Lactobacillus in the control group was significantly lower than that in the experimental group (all P<0.05).
Conclusion The changes of intestinal flora in children with ADHD and obesity may provide reference for the treatment of children with ADHD.
摘要：目的 探究注意缺陷多动障碍 (ADHD) 合并肥胖患儿发病过程中肠道菌群特点。 方法 选取 2018 年 8 月 一2019 年 10 月期间杭州市第七人民医院接诊的 64 例 ADHD 患儿作为本次研究对象, 根据体重指数将其分为 正常体重 ADHD 患儿(对照组, 32 例)和肥胖伴 ADHD 患儿(观察组, 32 例), 采用宏基因组测序的方法测定粪便 菌群, 比较 2 组儿童之间菌群 α 多样性、物种分类的相对丰度及菌数量的差异。 结果 观察组和对照组儿童肠道 菌群 α 多样性差异较小(均 P>0.05)。从菌属方面来看, 观察组患儿的韦荣球菌属和粪肠杆菌属丰度低于对照 组, 但气味杆菌属和肠球菌属丰度高于对照组(均 P<0.05)。从菌种方面来看, 观察组患儿的毛螺科菌、普氏栖 粪杆菌、小韦荣球菌以及活泼瘤胃球菌丰度低于对照组, 木假单胞菌、粪拟杆菌以及内脏拟杆菌丰度高于对照组 (均 P<0.05)。对照组患儿的直肠真杆菌菌数明显高于观察组, 多形拟杆菌、双歧杆菌属以及乳酸杆菌菌数明显 低于观察组(均 P<0.05)。 结论 ADHD 合并肥胖患儿肠道菌群发生了改变, 或可为 ADHD 患儿治疗提供参考 资料。