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      Transcriptomic Profiling of the Immune Response to Crowding Stress in Juvenile Turbot ( Scophthalmus maximus)

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          Abstract

          In this study, juvenile turbot Scophthalmus maximus were vaccinated with attenuated Edwardsiella tarda (EIBAV1) and reared at two different densities, low density (LD), (5.25 ± 0.02) kg m -2, as control group and high density (HD), (20.53 ± 0.05) kg m -2, as experimental group. Only density was considered as the variable. Five weeks after vaccination, the transcriptomes of spleen and head kidney from the turbot in two groups were analyzed with RNA-Seq technology. A total of 447 million reads were assembled into 41136 genes with an average length of 1274 bp and a N 50 size of 2295 bp. A comparison of gene expression between HD and LD groups revealed 1155 differentially expressed genes (DEGs). Enrichment and pathway analysis of the 10 immune-related DEGs showed the centrality of toll-like receptor signaling pathway, cytosolic DNA-sensing pathway and platelet activation in the host immune responses. The 5 overexpressed inflammatory cytokines and 5 downregulated signal-regulated cytokines genes are covered by these immune-related DEGs. It was inferred that cells suffer damage and the immune response is restrained in turbot under crowding stress.

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          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          10 July 2020
          01 August 2020
          : 19
          : 4
          : 911-922
          Affiliations
          1Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
          2Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
          3College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China
          Author notes
          *Corresponding author: LIU Baoliang, E-mail: Liubl@ 123456ysfri.ac.cn
          Article
          s11802-020-4242-6
          10.1007/s11802-020-4242-6
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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          Self URI (journal-page): https://www.springer.com/journal/11802

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