Transcriptomic Profiling of the Immune Response to Crowding Stress in Juvenile Turbot ( Scophthalmus maximus)

Abstract

In this study, juvenile turbot Scophthalmus maximus were vaccinated with attenuated Edwardsiella tarda (EIBAV1) and reared at two different densities, low density (LD), (5.25 ± 0.02) kg m ^-2, as control group and high density (HD), (20.53 ± 0.05) kg m ^-2, as experimental group. Only density was considered as the variable. Five weeks after vaccination, the transcriptomes of spleen and head kidney from the turbot in two groups were analyzed with RNA-Seq technology. A total of 447 million reads were assembled into 41136 genes with an average length of 1274 bp and a N ₅₀ size of 2295 bp. A comparison of gene expression between HD and LD groups revealed 1155 differentially expressed genes (DEGs). Enrichment and pathway analysis of the 10 immune-related DEGs showed the centrality of toll-like receptor signaling pathway, cytosolic DNA-sensing pathway and platelet activation in the host immune responses. The 5 overexpressed inflammatory cytokines and 5 downregulated signal-regulated cytokines genes are covered by these immune-related DEGs. It was inferred that cells suffer damage and the immune response is restrained in turbot under crowding stress.

Journal

Journal ID (publisher-id): JOUC

Title: Journal of Ocean University of China

Publisher: Science Press and Springer (China )

ISSN (Electronic): 1672-5182

Publication date (Electronic): 10 July 2020

Publication date (Print): 01 August 2020

Volume: 19

Issue: 4

Pages: 911-922

Affiliations

¹Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

²Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China

³College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China

Author notes

*Corresponding author: LIU Baoliang, E-mail: Liubl@ 123456ysfri.ac.cn

Article

Publisher ID: s11802-020-4242-6

DOI: 10.1007/s11802-020-4242-6

Copyright statement: Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

License:

The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

Product

Self URI (journal-page): https://www.springer.com/journal/11802