15
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Full-field optical coherence tomography for the analysis of fresh unstained human lobectomy specimens

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Background:

          Full-field optical coherence tomography (FFOCT) is a real-time imaging technique that generates high-resolution three-dimensional tomographic images from unprocessed and unstained tissues. Lack of tissue processing and associated artifacts, along with the ability to generate large-field images quickly, warrants its exploration as an alternative diagnostic tool.

          Materials and Methods:

          One section each from the tumor and from adjacent non-neoplastic tissue was collected from 13 human lobectomy specimens. They were imaged fresh with FFOCT and then submitted for routine histopathology. Two blinded pathologists independently rendered diagnoses based on FFOCT images.

          Results:

          Normal lung architecture (alveoli, bronchi, pleura and blood vessels) was readily identified with FFOCT. Using FFOCT images alone, the study pathologists were able to correctly identify all tumor specimens and in many cases, the histological subtype of tumor (e.g., adenocarcinomas with various patterns). However, benign diagnosis was provided with high confidence in roughly half the tumor-free specimens (others were diagnosed as equivocal or false positive). Further analysis of these images revealed two major confounding features: (a) Extensive lung collapse and (b) presence of smoker's macrophages. On a closer inspection, however, the smoker's macrophages could often be identified as distinct from tumor cells based on their relative location in the alveoli, size and presence of anthracosis. We posit that greater pathologist experience, complemented with morphometric analysis and color-coding of image components, may help minimize the contribution of these confounders in the future.

          Conclusion:

          Our study provides evidence for the potential utility of FFOCT in identifying and differentiating lung tumors from non-neoplastic lung tissue. We foresee its potential as an adjunct to intra-surgical frozen section analysis for margin assessment, especially in limited lung resections.

          Related collections

          Most cited references23

          • Record: found
          • Abstract: found
          • Article: not found

          High-resolution full-field optical coherence tomography with a Linnik microscope.

          We describe an original microscope for high-resolution optical coherence tomography applications. Our system is based on a Linnik interference microscope with high-numerical-aperture objectives. Lock-in detection of the interference signal is achieved in parallel on a CCD by use of a photoelastic birefringence modulator and full-field stroboscopic illumination with an infrared LED. Transverse cross-section (en-face, or XY) images can be obtained in real time with better than 1-microm axial (Z) resolution and 0.5-microm transverse (XY) resolution. A sensitivity of approximately 80 dB is reached at a 1-image/s acquisition rate, which allows tomography in scattering media such as biological tissues.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: found
            Is Open Access

            Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography

            We demonstrate the use of a high resolution form of optical coherence tomography, termed micro-OCT (μOCT), for investigating the functional microanatomy of airway epithelia. μOCT captures several key parameters governing the function of the airway surface (airway surface liquid depth, periciliary liquid depth, ciliary function including beat frequency, and mucociliary transport rate) from the same series of images and without exogenous particles or labels, enabling non-invasive study of dynamic phenomena. Additionally, the high resolution of μOCT reveals distinguishable phases of the ciliary stroke pattern and glandular extrusion. Images and functional measurements from primary human bronchial epithelial cell cultures and excised tissue are presented and compared with measurements using existing gold standard methods. Active secretion from mucus glands in tissue, a key parameter of epithelial function, was also observed and quantified.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Ultrahigh-resolution full-field optical coherence tomography.

              We have developed a white-light interference microscope for ultrahigh-resolution full-field optical coherence tomography of biological media. The experimental setup is based on a Linnik-type interferometer illuminated by a tungsten halogen lamp. En face tomographic images are calculated by a combination of interferometric images recorded by a high-speed CCD camera. Spatial resolution of 1.8 microm x 0.9 microm (transverse x axial) is achieved owing to the extremely short coherence length of the source, the compensation of dispersion mismatch in the interferometer arms, and the use of relatively high-numerical-aperture microscope objectives. A shot-noise-limited detection sensitivity of 90 dB is obtained in an acquisition time per image of 4 s. Subcellular-level images of plant, animal, and human tissues are presented.
                Bookmark

                Author and article information

                Contributors
                Journal
                J Pathol Inform
                J Pathol Inform
                JPI
                Journal of Pathology Informatics
                Medknow Publications & Media Pvt Ltd (India )
                2229-5089
                2153-3539
                2013
                27 September 2013
                : 4
                : 26
                Affiliations
                [1 ]Department of Urology, Weill Medical College of Cornell University, New York, USA
                [2 ]Department of Biochemistry, Weill Medical College of Cornell University, New York, USA
                [3 ]Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, USA
                [4 ]Department of Cardiothoracic Surgery, Weill Medical College of Cornell University, New York, USA
                [5 ]Langevin Institute, at ESPCI (1 rue Jussieu, 75005 Paris, France)
                [6 ]LLTech, at Cochin Hospital's Business Incubator (29 rue du Faubourg Saint-Jacques, 75014 Paris, France)
                [# ]Department of Science, Borough of Manhattan Community College, New York, NY, US
                Author notes
                [* ]Corresponding author
                Article
                JPI-4-26
                10.4103/2153-3539.119004
                3814996
                24244883
                de220d19-316b-4fc4-b96e-5e801aba5cc9
                Copyright: © 2013 Jain M

                This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 23 May 2013
                : 27 July 2013
                Categories
                Original Article

                Pathology
                full-field optical coherence tomography,histopathology,lobectomy,neoplastic tissue
                Pathology
                full-field optical coherence tomography, histopathology, lobectomy, neoplastic tissue

                Comments

                Comment on this article