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      ACTH Regulation of Adrenal mRNA Coding for Total and Specific Adrenal Proteins

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          The effects in vivo ACTH administration on the synthesis of mRNA coding for total adrenal proteins and for protein E a specific marker of ACTH action, have been studied. After 4 h of in vivo ACTH treatment, protein E is one of the major translational products. Its electrophoretic characteristics in a 2D gel acrylamide system are defined (molecular mass = 36,000 daltons, pHi = 7). We have investigated the effects of ACTH on both poly(A)-RNA coding for total adrenal proteins, and non-poly(A)-RNA. The time course of these effects is different: the effect on mRNA is maximal at 48 h whereas the effect on non-poly(A)-RNA continues to increase until the end of the experiment (5 days). In vitro translocational assays of mRNA indicate that the highest efficiency (protein synthesis/µg of mRNA) is observed after 4 h of ACTH treatment in vivo. After 5 days this efficiency is similar to that of mRNA extracted from non ACTH-treated rats.

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          Author and article information

          Horm Res Paediatr
          Hormone Research in Paediatrics
          S. Karger AG
          28 November 2008
          : 23
          : 1
          : 50-59
          Inserm, U-162, Hôpital Debrousse, Lyon, France
          180287 Horm Res 1986;23:50–59
          © 1986 S. Karger AG, Basel

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          Pages: 10


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