7
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Clinical Application of 25-MHz Ultrasound Biomicroscopy for Lens Opacity Degree Measurements in Phacoemulsification

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Purpose

          To evaluate the correlation between lens opacity degrees and phacoemulsification cumulated dissipated energy (CDE) values in patients with age-related cataract by applying 25-MHz panoramic ultrasound biomicroscopy (UBM).

          Methods

          This observational study was conducted in 227 patients (294 eyes) with age-related cataract. Patient ages ranged from 45 to 91 years. According to the lens images acquired by 25-MHz UBM, the objective indexes of lens opacity degrees were determined by using the ImageJ software. The correlation between lens opacity degrees (pixel units) and CDE values of phacoemulsification were mainly analyzed.

          Results

          The means of measurements were as follows: preoperative LogMAR corrected distance visual acuity, anterior chamber depth, and central lens thickness was 1.10 ± 0.61, 2.52 ± 0.51, and 4.34 ± 0.60 mm, respectively. The mean 25-MHz UBM-measured lens opacity degree was 101.30 ± 19.70 pixel units, and the mean CDE value was 9.74 ± 9.10. There was a linear correlation between pixel units and the CDE value, as well as LogMAR corrected distance visual acuity ( r = 0.38 and 0.50, respectively; both P < 0.05). Age correlated with anterior chamber depth and lens thickness ( r = −0.18 and 0.16, respectively; both P < 0.05) but not with pixel units ( r = −0.08, P > 0.05).

          Conclusion

          The 25-MHz UBM has significant advantages in displaying the opacity feature of age-related cataract. The 25-MHz UBM combined with ImageJ software can be used to evaluate the opacity degree of age-related cataract quantitatively and may help predict the phacoemulsification parameters in cataract surgery.

          Translational Relevance

          Combination of the lens ultrasonic image and image analysis software enables researchers to evaluate lens opacity degree quantitatively and predict the parameters of phacoemulsification surgery.

          Related collections

          Most cited references17

          • Record: found
          • Abstract: found
          • Article: not found

          Ageing and vision: structure, stability and function of lens crystallins.

          The alpha-, beta- and gamma-crystallins are the major protein components of the vertebrate eye lens, alpha-crystallin as a molecular chaperone as well as a structural protein, beta- and gamma-crystallins as structural proteins. For the lens to be able to retain life-long transparency in the absence of protein turnover, the crystallins must meet not only the requirement of solubility associated with high cellular concentration but that of longevity as well. For proteins, longevity is commonly assumed to be correlated with long-term retention of native structure, which in turn can be due to inherent thermodynamic stability, efficient capture and refolding of non-native protein by chaperones, or a combination of both. Understanding how the specific interactions that confer intrinsic stability of the protein fold are combined with the stabilizing effect of protein assembly, and how the non-specific interactions and associations of the assemblies enable the generation of highly concentrated solutions, is thus of importance to understand the loss of transparency of the lens with age. Post-translational modification can have a major effect on protein stability but an emerging theme of the few studies of the effect of post-translational modification of the crystallins is one of solubility and assembly. Here we review the structure, assembly, interactions, stability and post-translational modifications of the crystallins, not only in isolation but also as part of a multi-component system. The available data are discussed in the context of the establishment, the maintenance and finally, with age, the loss of transparency of the lens. Understanding the structural basis of protein stability and interactions in the healthy eye lens is the route to solve the enormous medical and economical problem of cataract.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Correlation of nuclear cataract lens density using Scheimpflug images with Lens Opacities Classification System III and visual function.

            To calculate the average lens density (ALD) and nuclear lens density (NLD) using Scheimpflug images and to determine their correlation with logarithmic minimal angle resolution (logMAR) best-corrected visual acuity (BCVA), contrast sensitivity (CS), and lens grading based on the Lens Opacities Classification System (LOCS) III.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Advances in ultrasound biomicroscopy.

              The visualisation of living tissues at microscopic resolution is attracting attention in several fields. In medicine, the goals are to image healthy and diseased tissue with the aim of providing information previously only available from biopsy samples. In basic biology, the goal may be to image biological models of human disease or to conduct longitudinal studies of small-animal development. High-frequency ultrasonic imaging (ultrasound biomicroscopy) offers unique advantages for these applications. In this paper, the development of ultrasound biomicroscopy is reviewed. Aspects of transducer development, systems design and tissue properties are presented to provide a foundation for medical and biological applications. The majority of applications appear to be developing in the 40-60-MHz frequency range, where resolution on the order of 50 microm can be achieved. Doppler processing in this frequency range is beginning to emerge and some examples of current achievements will be highlighted. The current state of the art is reviewed for medical applications in ophthalmology, intravascular ultrasound, dermatology, and cartilage imaging. Ultrasound biomicroscopic studies of mouse embryonic development and tumour biology are presented. Speculation on the continuing evolution of ultrasound biomicroscopy will be discussed.
                Bookmark

                Author and article information

                Journal
                Transl Vis Sci Technol
                Transl Vis Sci Technol
                tvst
                Transl Vis Sci Technol
                TVST
                Translational Vision Science & Technology
                The Association for Research in Vision and Ophthalmology
                2164-2591
                July 2019
                7 August 2019
                : 8
                : 4
                : 18
                Affiliations
                [1 ]Department of Ophthalmology, The Fourth Affiliated Hospital of China Medical University, Eye Hospital of China Medical University, The Key Laboratory of Lens in Liaoning Province, Shenyang, China
                Author notes
                Correspondence: Mingyu Shi, No.11 Xinhua Road, HePing District, Shenyang, Liaoning, China. e-mail: myshi@ 123456cmu.edu.cn
                Article
                tvst-08-04-15 TVST-18-1246
                10.1167/tvst.8.4.18
                6685485
                de956ac3-1cfd-4c27-b7ac-1e51f2aecd6c
                Copyright 2019 The Authors

                This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                History
                : 19 November 2018
                : 10 June 2019
                Categories
                Articles

                25-mhz ubm,age-related cataract,cde,phacoemulsification,imagej

                Comments

                Comment on this article