17
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Effects of a Closed Space Environment on Gene Expression in Hair Follicles of Astronauts in the International Space Station

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Adaptation to the space environment can sometimes pose physiological problems to International Space Station (ISS) astronauts after their return to earth. Therefore, it is important to develop healthcare technologies for astronauts. In this study, we examined the feasibility of using hair follicles, a readily obtained sample, to assess gene expression changes in response to spaceflight adaptation. In order to investigate the gene expression changes in human hair follicles during spaceflight, hair follicles of 10 astronauts were analyzed by microarray and real time qPCR analyses. We found that spaceflight alters human hair follicle gene expression. The degree of changes in gene expression was found to vary among individuals. In some astronauts, genes related to hair growth such as FGF18, ANGPTL7 and COMP were upregulated during flight, suggesting that spaceflight inhibits cell proliferation in hair follicles.

          Related collections

          Most cited references41

          • Record: found
          • Abstract: not found
          • Article: not found

          The biology of hair follicles.

            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            The secret life of the hair follicle.

            M. Hardy (1992)
            The mammalian hair follicle is a treasure waiting to be discovered by more molecular geneticists. How can a tiny cluster of apparently uniform epithelial cells, adjacent to a tiny cluster of uniform mesenchymal cells, give rise to five or six concentric cylinders, each of which is composed of cells of a distinctive type that synthesize their own distinctive set of proteins? There is now evidence that several growth factors, cell adhesion molecules and other molecules play important roles in the regulation of this minute organ.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Controls of hair follicle cycling.

              K Stenn, R Paus (2001)
              Nearly 50 years ago, Chase published a review of hair cycling in which he detailed hair growth in the mouse and integrated hair biology with the biology of his day. In this review we have used Chase as our model and tried to put the adult hair follicle growth cycle in perspective. We have tried to sketch the adult hair follicle cycle, as we know it today and what needs to be known. Above all, we hope that this work will serve as an introduction to basic biologists who are looking for a defined biological system that illustrates many of the challenges of modern biology: cell differentiation, epithelial-mesenchymal interactions, stem cell biology, pattern formation, apoptosis, cell and organ growth cycles, and pigmentation. The most important theme in studying the cycling hair follicle is that the follicle is a regenerating system. By traversing the phases of the cycle (growth, regression, resting, shedding, then growth again), the follicle demonstrates the unusual ability to completely regenerate itself. The basis for this regeneration rests in the unique follicular epithelial and mesenchymal components and their interactions. Recently, some of the molecular signals making up these interactions have been defined. They involve gene families also found in other regenerating systems such as fibroblast growth factor, transforming growth factor-beta, Wnt pathway, Sonic hedgehog, neurotrophins, and homeobox. For the immediate future, our challenge is to define the molecular basis for hair follicle growth control, to regenerate a mature hair follicle in vitro from defined populations, and to offer real solutions to our patients' problems.
                Bookmark

                Author and article information

                Contributors
                Role: Editor
                Journal
                PLoS One
                PLoS ONE
                plos
                plosone
                PLoS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                30 March 2016
                2016
                : 11
                : 3
                : e0150801
                Affiliations
                [1 ]Divison of Aerospace Medicine, The Jikei University School of Medicine, Minato-ku, Tokyo, Japan
                [2 ]Japan Aerospace Exploration Agency, Tsukuba City, Ibaraki, Japan
                [3 ]Space Biosciences Division, NASA Ames Research Center, Moffett Field, California, United States of America
                [4 ]Advanced Engineering Services Co., Ltd., Takezono, Tsukuba City, Ibaraki, Japan
                [5 ]Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima City, Kagoshima, Japan
                [6 ]Institute of Space and Astronautical Science, Sagamihara, Kanagawa, Japan
                [7 ]Department of Space and Astronautical Science, School of Physical Sciences, SOKENDAI (The Graduate University for Advanced Studies), Sagamihara, Kanagawa, Japan
                Texas Tech University, UNITED STATES
                Author notes

                Competing Interests: The experimental cost for this study was funded by Japan Aerospace Exploration Agency (JAXA). Masaya Seki and Rika Takahashi are employed by Advanced Engineering Services Co., Ltd. There are no patents, products in development, or marketed products to declare. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors.

                Conceived and designed the experiments: MT SY AH HM M. Sudoh CM NI. Performed the experiments: MT M. Seki RT. Analyzed the data: MT. Contributed reagents/materials/analysis tools: MT. Wrote the paper: MT.

                Article
                PONE-D-15-38862
                10.1371/journal.pone.0150801
                4814050
                27029003
                df578ba5-18f1-4858-bfa8-706feb2cde64
                © 2016 Terada et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 2 September 2015
                : 19 February 2016
                Page count
                Figures: 6, Tables: 3, Pages: 17
                Funding
                The experimental cost for this study was funded by Japan Aerospace Exploration Agency (JAXA). Advanced Engineering Services Co., Ltd. provided support in the form of salaries for authors MS (2nd author) and RT, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section.
                Categories
                Research Article
                Biology and life sciences
                Biochemistry
                Nucleic acids
                RNA
                Messenger RNA
                Biology and Life Sciences
                Anatomy
                Integumentary System
                Hair
                Hair Follicles
                Medicine and Health Sciences
                Anatomy
                Integumentary System
                Hair
                Hair Follicles
                Biology and Life Sciences
                Anatomy
                Integumentary System
                Skin
                Hair Follicles
                Medicine and Health Sciences
                Anatomy
                Integumentary System
                Skin
                Hair Follicles
                Biology and Life Sciences
                Anatomy
                Integumentary System
                Hair
                Medicine and Health Sciences
                Anatomy
                Integumentary System
                Hair
                Biology and Life Sciences
                Genetics
                Gene Expression
                Physical Sciences
                Astronomical Sciences
                Space Exploration
                Spaceflight
                People and Places
                Population Groupings
                Professions
                Astronauts
                Research and Analysis Methods
                Bioassays and Physiological Analysis
                Microarrays
                Research and analysis methods
                Extraction techniques
                RNA extraction
                Custom metadata
                All relevant data are within the paper. All raw fluorescence intensity data and microarray image files were deposited within the public repository for microarray-based gene expression data, the “Gene Expression Omnibus” (GEO) ( http://www.ncbi.nlm.nih.gov/geo/), complying with the minimum information requirement for microarray experiments. The GEO accession number for the current experiment is GSE74708.

                Uncategorized
                Uncategorized

                Comments

                Comment on this article