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      Single-locus DNA barcoding and species delimitation of the sandfly subgenus Evandromyia (Aldamyia).

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          Abstract

          Sandfly specimens from the subgenus Evandromyia (Aldamyia) Galati, 2003 (Diptera: Psychodidae: Phlebotominae) were collected between 2012 and 2019 from nine localities in seven Brazilian states, morphologically-identified, and then DNA barcoded by sequencing the mitochondrial cytochrome c oxidase subunit I (coi) gene. Forty-four new barcode sequences generated from 10 morphospecies were combined with 49 previously published sequences from the same subgenus and analysed using sequence-similarity methods (best-match criteria) to assess their ability at specimen identification, while four different species delimitation methods (ABGD, GMYC, PTP and TCS) were used to infer molecular operational taxonomic units (MOTUs). Overall, seven of the 11 morphospecies analysed were congruent with both the well-supported clades identified by phylogenetic analysis and the MOTUs inferred by species delimitation, while the remaining four morphospecies - E. carmelinoi, E. evandroi, E. lenti and E. piperiformis - were merged into a single well-supported clade/MOTU. Although E. carmelinoi, E. evandroi and E. lenti were indistinguishable using coi DNA barcodes, E. piperiformis did form a distinct phylogenetic cluster and could be correctly identified using best-match criteria. Despite their apparent morphological differences, we propose on the basis of the molecular similarity of their DNA barcodes that these latter four morphospecies should be considered members of a recently-diverged species complex.

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          Author and article information

          Journal
          Med Vet Entomol
          Medical and veterinary entomology
          Wiley
          1365-2915
          0269-283X
          Dec 2020
          : 34
          : 4
          Affiliations
          [1 ] Grupo de Estudos em Leishmanioses, Instituto René Rachou-Fiocruz Minas, Belo Horizonte, Minas Gerais, Brazil.
          [2 ] 50 Rowntree Way Saffron Walden, Essex, CB11 4DL, U.K.
          [3 ] Coleção de Flebotomíneos (FIOCRUZ/COLFLEB), Instituto René Rachou-Fiocruz Minas, Belo Horizonte, Minas Gerais, Brazil.
          Article
          10.1111/mve.12458
          32590879
          dfc392fe-5148-43d7-a61d-58a8a1eb66f0
          History

          species complex,taxonomy,species delimitation,sandfly,phylogenetics,coi,Phlebotominae,Evandromyia,DNA barcodes,Brazil

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