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      Data on the construction of a recombinant HEK293 cell line overexpressing hERG potassium channel and examining the presence of hERG mRNA and protein expression

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          Abstract

          The data presented in this article are related to the research article entitled “The effects of deoxyelephantopin on the cardiac delayed rectifier potassium channel current (I Kr) and human ether-a-go-go-related gene (hERG) expression” (Y.F. Teah, M.A. Abduraman, A. Amanah, M.I. Adenan, S.F. Sulaiman, M.L. Tan) [1], which the possible hERG blocking properties of deoxyelephantopin were investigated. This article describes the construction of human embryonic kidney 293 (HEK293) cells overexpressing HERG potassium channel and verification of the presence of hERG mRNA and protein expression in this recombinant cell line.

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          The effects of deoxyelephantopin on the cardiac delayed rectifier potassium channel current (IKr) and human ether-a-go-go-related gene (hERG) expression.

          Yi Teah, Muhammad Asyraf Abduraman, Azimah Amanah … (2017)
          Elephantopus scaber Linn and its major bioactive component, deoxyelephantopin are known for their medicinal properties and are often reported to have various cytotoxic and antitumor activities. This plant is widely used as folk medicine for a plethora of indications although its safety profile remains unknown. Human ether-a-go-go-related gene (hERG) encodes the cardiac IKr current which is a determinant of the duration of ventricular action potentials and QT interval. The hERG potassium channel is an important antitarget in cardiotoxicity evaluation. This study investigated the effects of deoxyelephantopin on the current, mRNA and protein expression of hERG channel in hERG-transfected HEK293 cells. The hERG tail currents following depolarization pulses were insignificantly affected by deoxyelephantopin in the transfected cell line. Current reduction was less than 40% as compared with baseline at the highest concentration of 50 μM. The results were consistent with the molecular docking simulation and hERG surface protein expression. Interestingly, it does not affect the hERG expression at both transcriptional and translational level at most concentrations, although higher concentration at 10 μM caused protein accumulation. In conclusion, deoxyelephantopin is unlikely a clinically significant hERG channel and Ikr blocker.
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            Author and article information

            Contributors
            Mei Lan Tan
            Journal
            Journal ID (nlm-ta): Data Brief
            Journal ID (iso-abbrev): Data Brief
            Title: Data in Brief
            Publisher: Elsevier
            ISSN (Electronic): 2352-3409
            Publication date PMC-release: 12 August 2017
            Publication date Collection: October 2017
            Publication date (Electronic): 12 August 2017
            Volume: 14
            Pages: 584-591
            Affiliations
            [a ]Malaysian Institute of Pharmaceuticals & Nutraceuticals, National Institutes of Biotechnology Malaysia (NIBM), Ministry of Science, Technology and Innovation Malaysia, Pulau Pinang, Malaysia
            [b ]Advanced Medical & Dental Institute, Universiti Sains Malaysia, Pulau Pinang, Malaysia
            [c ]Atta-ur-Rahman Institute for Natural Product Discovery, Universiti Teknologi MARA (UiTM), Selangor Darul Ehsan, Malaysia
            [d ]School of Biological Sciences, Universiti Sains Malaysia, Pulau Pinang, Malaysia
            Author notes
            [* ]Correspondence to: Lifestyle Science Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Bertam, Kepala Batas, Pulau Pinang, Malaysia. Tel : 604-5622309, Fax : 604-5622349. tanml@ 123456usm.my
            Article
            Publisher ID: S2352-3409(17)30385-2
            DOI: 10.1016/j.dib.2017.08.008
            PMC ID: 5574817
            PubMed ID: 28879216
            SO-VID: e001aacb-5eed-42fa-943f-1aebda6cdcfa
            Copyright © © 2017 The Authors
            License:

            This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

            History
            Date received : 7 July 2017
            Date revision received : 31 July 2017
            Date accepted : 3 August 2017
            Categories
            Subject: Data Article

            Keywords: herg,recombinant cell line,herg mrna expression,herg protein expression
            Data availability:
            Keywords: herg, recombinant cell line, herg mrna expression, herg protein expression

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