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      Actin genes and their expression in pacific white shrimp, Litopenaeus vannamei.

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          Abstract

          Actin is a multi-functional gene family that can be divided into muscle-type actins and non-muscle-type actins. In this study, 37 unigenes encoding actins were identified from RNA-Seq data of Pacific white shrimp, Litopenaeus vannamei. According to phylogenetic analysis, four and three cDNAs belong to cytoplasmic- and heart-type actins and were named LvActinCT and LvActinHT, respectively. 10 cDNAs belong to the slow-type skeletal muscle actins, and 18 belong to the fast-type skeletal muscle actins; they were designated LvActinSSK and LvActinFSK, respectively. Some muscle actin genes formed gene clusters in the genome. Multiple alternative transcription starts sites (ATSSs) were found for LvActinCT1. Based on the early developmental expression profile, almost all LvActins were highly expressed between the early limb bud and post-larval stages. Using LvActinSSK5 as probes, slow-type muscle was localized in pleopod muscle and superficial ventral muscle. We also found three actin genes that were down-regulated in the hemocytes of white spot syndrome virus (WSSV)- and Vibrio parahaemolyticus-infected L. vannamei. This study provides valuable information on the actin gene structure of shrimp, furthers our understanding of the shrimp muscle system and helps us develop strategies for disease control and sustainable shrimp farming.

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          Author and article information

          Journal
          Mol. Genet. Genomics
          Molecular genetics and genomics : MGG
          Springer Science and Business Media LLC
          1617-4623
          1617-4623
          Apr 2018
          : 293
          : 2
          Affiliations
          [1 ] Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China. xiaoxizhang11@gmail.com.
          [2 ] Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China. xiaoxizhang11@gmail.com.
          [3 ] University of Chinese Academy of Sciences, Beijing, 100049, China. xiaoxizhang11@gmail.com.
          [4 ] Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
          [5 ] Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
          [6 ] University of Chinese Academy of Sciences, Beijing, 100049, China.
          Article
          10.1007/s00438-017-1397-y
          10.1007/s00438-017-1397-y
          29189957
          e033aa47-9b23-4cc1-9957-110bdd577041
          History

          RNA-Seq,Actin gene,Disease control,Litopenaeus vannamei,Muscle development

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