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      X-ray structure of Arthrobacter globiformis M30 ketose 3-epimerase for the production of d-allulose from d-fructose

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          Abstract

          The crystal structure of A. globiformis M30 ketose 3-epimerase was determined at 1.96 Å resolution.

          Abstract

          The X-ray structure of ketose 3-epimerase from Arthrobacter globiformis M30, which was previously reported to be a d-allulose 3-epimerase (AgD-AE), was determined at 1.96 Å resolution. The crystal belonged to the hexagonal space group P6 522, with unit-cell parameters a = b = 103.98, c = 256.53 Å. The structure was solved by molecular replacement using the structure of Mesorhizobium loti l-ribulose 3-epimerase (MlL-RE), which has 41% sequence identity, as a search model. A hexagonal crystal contained two molecules in the asymmetric unit, and AgD-AE formed a homotetramer with twofold symmetry. The overall structure of AgD-AE was more similar to that of MlL-RE than to the known structures of d-psicose (alternative name d-allulose) 3-epimerases ( d-PEs or d-AEs), although AgD-AE and MlL-RE have different substrate specificities. Both AgD-AE and MlL-RE have long helices in the C-terminal region that would contribute to the stability of the homotetramer. AgD-AE showed higher enzymatic activity for l-ribulose than d-allulose; however, AgD-AE is stable and is a unique useful enzyme for the production of d-allulose from d-fructose.

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          Author and article information

          Journal
          Acta Crystallogr F Struct Biol Commun
          Acta Crystallogr F Struct Biol Commun
          Acta Cryst. F
          Acta Crystallographica. Section F, Structural Biology Communications
          International Union of Crystallography
          2053-230X
          01 October 2018
          21 September 2018
          01 October 2020
          : 74
          : Pt 10 ( publisher-idID: f181000 )
          : 669-676
          Affiliations
          [a ]Life Science Research Center and Faculty of Medicine, Kagawa University , 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan
          [b ]International Institute of Rare Sugar Research and Education, Kagawa University , Kagawa, Japan
          [c ]Faculty of Agriculture, Kagawa University , 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0795, Japan
          [d ] Matsutani Chemical Industry Co. Ltd , 5-3 Kita-Itami, Itami, Hyogo 664-8508, Japan
          Author notes
          Article
          PMC6168773 PMC6168773 6168773 no5140 ACSFEN S2053230X18011706
          10.1107/S2053230X18011706
          6168773
          30279320
          e07e3f19-4e24-4b74-a68e-6cd18ab8e548
          © International Union of Crystallography 2018

          Prior permission is not required to reproduce short quotations, tables and figures from this article, provided the original authors and source are cited. For more information, see http://journals.iucr.org/services/copyrightpolicy.html.

          History
          : 13 March 2018
          : 19 August 2018
          Page count
          Pages: 8
          Funding
          Funded by: Japan Society for the Promotion of Science
          Award ID: KAKENHI 16K07271
          This work was funded by Japan Society for the Promotion of Science grant KAKENHI 16K07271.
          Categories
          Research Communications

          X-ray structure, d-allulose,β/α-barrel,ketose 3-epimerase, Arthrobacter globiformis

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