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      Mitogen-activated protein kinase cascade in the signaling for polyamine biosynthesis in tobacco.

      Plant and Cell Physiology

      Carboxy-Lyases, metabolism, Gene Expression Regulation, Plant, MAP Kinase Kinase 2, genetics, MAP Kinase Signaling System, Mitogen-Activated Protein Kinases, Plant Proteins, Plants, Genetically Modified, Polyamines, Tobacco, enzymology, Transcription, Genetic

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          Abstract

          Expression of NtNEK2(DD), a constitutively active mutant of NtMEK2, activates endogenous salicylic acid-induced protein kinase (SIPK) and wounding-induced protein kinase (WIPK), and leads to several stress/defense responses in tobacco. In this study, we used ACP (annealing control primer)-based differential display reverse transcription-PCR to isolate the downstream effectors mediated by the NtMEK2-SIPK/WIPK cascade. The arginine decarboxylase gene (ADC), which is involved in plant putrescine biosynthesis, was one of nine differentially expressed genes. When compared with NtMEK2(KR) plants, NtMEK2(DD) transgenic plants exhibited a significant increase in ADC and ODC (ornithine decarboxylase) transcript levels, as well as in putrescine and its catabolite, gamma-aminobutyric acid, following SIPK/WIPK activation. Taken together, these results suggest that the NtMEK2-SIPK/WIPK cascade is involved in regulating polyamine synthesis, especially putrescine synthesis, through transcriptional regulation of the biosynthetic genes in tobacco.

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          Journal
          19151070
          10.1093/pcp/pcp009

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