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      Polymerase complex with lysine at position 627 of the PB2 of influenza virus A/Hong Kong/483/97 (H5N1) efficiently transcribes and replicates virus genes in mouse cells.

      1 , , ,
      Virus research
      Elsevier BV
      H5N1, Influenza A virus, PB2, Polymerase

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          Abstract

          Influenza virus A/Hong Kong/483/97 (H5N1) (HK483-K) has the PB2 with lysine at position 627 (PB2-627K) and is highly pathogenic in chickens and mice. On the other hand, the pathogenicity of mutant virus (HK483-E), which was generated by substituting lysine with glutamic acid at the position of the PB2, is lower than that of HK483-K in mice, but is highly pathogenic in chickens. The PB2 is one of the components of heterotrimeric polymerase complex, which plays roles in the transcription and replication of virus genes. Cell-free polymerase assay revealed that intrinsic transcription activity of the polymerase complex with PB2-627K is higher than that of glutamic acid (PB2-627E). In chicken cells, transcription efficiency of the polymerase complex with PB2-627E was not lower than those with PB2-627K, indicating that transcription of virus genes is modulated by some host factors in chicken cells, resulting in high growth. Polymerase complex with PB2-627K efficiently transcribes and replicates virus polymerase genes in mouse cells, leading to high growth of HK483-K compared with that of HK483-E. The results of our experiments clearly suggest that efficient transcription and replication of virus genes by polymerase complex result in the higher pathogenicity in mice.

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          Author and article information

          Journal
          Virus Res.
          Virus research
          Elsevier BV
          1872-7492
          0168-1702
          Dec 26 2013
          : 178
          : 2
          Affiliations
          [1 ] Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan.
          Article
          S0168-1702(13)00304-3
          10.1016/j.virusres.2013.09.012
          24070983
          e0f8b2f1-1857-4898-abbb-032c1956b7d8
          History

          Influenza A virus,H5N1,PB2,Polymerase
          Influenza A virus, H5N1, PB2, Polymerase

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