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      Lysosomal and Non-Lysosomal Pathways of Intracellular Insulin Degradation in Isolated Rat Hepatocytes

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          The amount of <sup>125</sup>I-insulin associated with freshly isolated hepatocytes was increased 50% in the presence of 0.2 m M chloroquine (CQ) after 2 h of incubation. The degradation of insulin by the hepatocytes incubated with CQ was significantly diminished as compared with control cells. Hepatocytes incubated with <sup>125</sup>I-insulin in the presence of CQ showed a slower rate of ligand dissociation than control cells. More TCA-precipitable and less TCA-soluble material appeared in the dissociation buffer of CQ-treated cells. However, CQ inhibited only 25-35% of intracellular insulin degradation. Non-lysosomal intracellular insulin degradation appears to be responsible for the remaining portion of the ligand degradation by isolated hepatocytes.

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          Author and article information

          Horm Res Paediatr
          Hormone Research in Paediatrics
          S. Karger AG
          25 November 2008
          : 15
          : 4
          : 252-262
          Research Service, Veterans Administration Medical Center, and the Division of Endocrinology, Department of Medicine, University of Colorado Health Sciences Center, Denver, Colo., USA
          179464 Horm Res 1981;15:252–262
          © 1981 S. Karger AG, Basel

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          Pages: 11
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