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      Lysosomal and Non-Lysosomal Pathways of Intracellular Insulin Degradation in Isolated Rat Hepatocytes

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          Abstract

          The amount of <sup>125</sup>I-insulin associated with freshly isolated hepatocytes was increased 50% in the presence of 0.2 m M chloroquine (CQ) after 2 h of incubation. The degradation of insulin by the hepatocytes incubated with CQ was significantly diminished as compared with control cells. Hepatocytes incubated with <sup>125</sup>I-insulin in the presence of CQ showed a slower rate of ligand dissociation than control cells. More TCA-precipitable and less TCA-soluble material appeared in the dissociation buffer of CQ-treated cells. However, CQ inhibited only 25-35% of intracellular insulin degradation. Non-lysosomal intracellular insulin degradation appears to be responsible for the remaining portion of the ligand degradation by isolated hepatocytes.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1981
          1981
          25 November 2008
          : 15
          : 4
          : 252-262
          Affiliations
          Research Service, Veterans Administration Medical Center, and the Division of Endocrinology, Department of Medicine, University of Colorado Health Sciences Center, Denver, Colo., USA
          Article
          179464 Horm Res 1981;15:252–262
          10.1159/000179464
          6765590
          © 1981 S. Karger AG, Basel

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          Page count
          Pages: 11
          Categories
          Original Paper

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