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Microinjections of L-Leucine into Tubules and Peritubular Capillaries of the Rat
Abstract
Antiluminal and luminal transport of amino acids has been studied in vivo with a microinjection technique in maleate-treated rats. Upon microinjections of l -leucine-<sup>3</sup>H and inulin-<sup>14</sup>C in the lumen of proximal convoluted tubules and in peritubular capillaries of the cortical segments of the nephron, the amount of radioactivity measured in ureteral urine gave an indication of amino acid absorption. The results suggest that the entry of amino acids into the renal tubule cells is not impeded in maleate-treated rats and that maleic acid does not act as a competitor for amino acid transport. After microinjections into peritubular capillaries of the left kidney, L-leucine excretion was found to be greater in this experimental kidney than in the contralateral non-injected kidney. However, in maleate-treated rats, the excretion of leucine preceded that of inulin in the experimental kidney in contrast to the non-injected kidney where leucine and inulin excretion occurred simultaneously. It is proposed that this modification of amino acid excretion can be explained by an enhanced transtubular flux of amino acid, caused by a decreased ability of the cell to retain absorbed amino acids.
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179939 Nephron 1971;8:367–374Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.