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      Developmental expression of B cell molecules in equine lymphoid tissues.

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          Abstract

          Identification and classification of B cell subpopulations has been shown to be challenging and inconsistent among different species. Our study tested aspects of ontogeny, phenotype, tissue distribution, and function of equine CD5hi B cells, which represented a greater proportion of B cells early in development and in the peritoneal cavity. CD5hi and CD5lo B cells differentially expressed B cell markers (CD2, CD21, IgM) measured using flow cytometry, but similar mRNA expression of signature genes (DGKA, FGL2, PAX5, IGHM, IL10) measured using quantitative RT-PCR. Sequencing lambda light chain segments revealed that CD5hi B cells generated diverse immunoglobulin repertoires, and more frequently bound to fluorescence-labeled phosphorylcholine. This study shows developmental characteristics and tissue distribution of a newly described subpopulation of B cells in the horse.

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          Author and article information

          Journal
          Vet. Immunol. Immunopathol.
          Veterinary immunology and immunopathology
          Elsevier BV
          1873-2534
          0165-2427
          Jan 2017
          : 183
          Affiliations
          [1 ] Equine Immunology Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. Electronic address: jmb279@cornell.edu.
          [2 ] Equine Immunology Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. Electronic address: rlt8@cornell.edu.
          [3 ] Equine Immunology Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. Electronic address: mbf6@cornell.edu.
          S0165-2427(16)30366-X NIHMS839134
          10.1016/j.vetimm.2016.12.004
          5267323
          28063478

          Immunoglobulin repertoire, Equine, Development, CD5, B cell

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