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      Intestinal Parasites in First World War German Soldiers from “Kilianstollen”, Carspach, France

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          Abstract

          Paleoparasitological investigations revealed the presence of intestinal helminths in samples taken from the abdominal cavities of two German soldiers, recovered in the First World War site named “Kilianstollen” in Carspach, France. Eggs from roundworm, whipworm, tapeworm and capillariids were identified. The morphological and morphometrical comparison, followed by statistical analyses, showed that the Carspach capillariid eggs are similar to rodent parasites. Poor sanitary conditions in the trenches, the lack of knowledge of parasites, and the widespread presence of commensal animals, can explain the occurrence of such parasites in human intestines. This study is the second dealing with 20 th century human samples. It confirms the presence of intestinal worms in First World War German soldiers. In this case study, the application of statistics to precise measurements facilitated the diagnosis of ancient helminth eggs and completed the microscopic approach.

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          Most cited references23

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          Intestinal capillariasis.

          Intestinal capillariasis caused by Capillaria philippinensis appeared first in the Philippines and subsequently in Thailand, Japan, Iran, Egypt, and Taiwan, but most infections occur in the Philippines and Thailand. As established experimentally, the life cycle involves freshwater fish as intermediate hosts and fish-eating birds as definitive hosts. Embryonated eggs from feces fed to fish hatch and grow as larvae in the fish intestines. Infective larvae fed to monkeys, Mongolian gerbils, and fish-eating birds develop into adults. Larvae become adults in 10 to 11 days, and the first-generation females produce larvae. These larvae develop into males and egg-producing female worms. Eggs pass with the feces, reach water, embryonate, and infect fish. Autoinfection is part of the life cycle and leads to hyperinfection. Humans acquire the infection by eating small freshwater fish raw. The parasite multiplies, and symptoms of diarrhea, borborygmus, abdominal pain, and edema develop. Chronic infections lead to malabsorption and hence to protein and electrolyte loss, and death results from irreversible effects of the infection. Treatment consists of electrolyte replacement and administration of an antidiarrheal agent and mebendazole or albendazole. Capillariasis philippinensis is considered a zoonotic disease of migratory fish-eating birds. The eggs are disseminated along flyways and infect the fish, and when fish are eaten raw, the disease develops.
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            The state of the art of paleoparasitological research in the Old World.

            Paleoparasitology in the Old World has mainly concerned the study of latrine sediments and coprolites collected from mummified bodies or archaeological strata, mostly preserved by natural conditions. Human parasites recovered include cestodes, trematodes, and nematodes. The well preserved conditions of helminth eggs allowed paleoepidemiological approaches taking into account the number of eggs found by archaeological stratum. Tentatively, sanitation conditions were assessed for each archaeological period.
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              Paleoparasitological records in a canid coprolite from Patagonia, Argentina.

              In this note, organic remains identified as a canid coprolite were examined. The material was dated at 6540 +/- 110 B.P.; it was collected in the Perito Moreno National Park, Santa Cruz, Argentina. Paleoparasitological analysis was performed following standard procedures. Coprolite fragments were rehydrated in a trisodium phosphate aqueous solution and subjected to spontaneous sedimentation for microscope analysis. Eggs of nematodes identified as Trichuris sp., Capillaria sp., Uncinaria sp., and an ascaridid (probably Toxascaris sp.) or spirurids (presumably Physaloptera sp.), plus a cestode (Anoplocephalidae), presumably Moniezia sp., were found.
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                Author and article information

                Contributors
                Role: Editor
                Journal
                PLoS One
                PLoS ONE
                plos
                plosone
                PLoS ONE
                Public Library of Science (San Francisco, USA )
                1932-6203
                2014
                15 October 2014
                : 9
                : 10
                : e109543
                Affiliations
                [1 ]University of Franche-Comte, CNRS UMR 6249 Chrono-Environment, Besancon, France
                [2 ]PAIR, CNRS UMR 7044 Archimède, ZA Sud, CIRSUD, Sélestat, France
                Universidade Federal de Minas Gerais, Brazil
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Performed the experiments: MLB. Analyzed the data: MLB LM BD. Wrote the paper: MLB ML LM BD. Excavated and provided the material for study: ML. Reviewed the bibliography: MLB BD.

                Article
                PONE-D-14-23929
                10.1371/journal.pone.0109543
                4198135
                e1506bfd-1747-4422-8eb0-ebc13dfce27e
                Copyright @ 2014

                This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 19 July 2014
                : 5 September 2014
                Page count
                Pages: 6
                Funding
                These authors have no support or funding to report.
                Categories
                Research Article
                Biology and Life Sciences
                Veterinary Science
                Veterinary Diseases
                Zoonoses
                Taeniasis
                Medicine and Health Sciences
                Infectious Diseases
                Parasitic Diseases
                Helminth Infections
                Soil-Transmitted Helminthiases
                Ascariasis
                Trichuriasis
                Nematode Infections
                Parasitic Intestinal Diseases
                Social Sciences
                Anthropology
                Archaeology
                Archaeobiology
                Archaeometry
                Custom metadata
                The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper and its Supporting Information files.

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                Uncategorized

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