1
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Compensatory Renal Hypertrophy: Tubular Cell Growth and Transport Studied in Primary Culture

      ,

      Nephron

      S. Karger AG

      Primary renal tubular cell culture, Tubular cell growth, Sodium transport

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          In order to define the growth and transport characteristics of renal epithelia during hypertrophy, primary culture of proximal tubular cells was established from kidneys of normal rats and those with compensatory renal hypertrophy (CRH) induced by unilateral nephrectomy. At confluence, enhanced growth potential was preserved in the cells derived from the animals with CRH (CRH cells) as compared with cells derived from normal animals (N cells), as evidenced by an increased cellular protein content (250 ± 12 vs. 151 ± 11 pg protein/cell; p < 0.001) and a somewhat lesser increase in cellular thymidine incorporation (6,820 ± 52 vs. 5,151 ± 308 cpm/10<sup>6</sup> cells; p < 0.02). The cellular Na transport (measured as <sup>22</sup>Na uptake) was increased in the CRH cells, being 733 ± 47, 886 ± Ill, and 1,026 ± 147 cpm/1,000 cells at 1,3, and 120 min, respectively, versus 353 ± 36,469 ± 31, and 549 ± 41 cpm/1,000 cells in N cells (p < 0.05 for each time point), i.e., an increase in Na transport in CRH cells of 107, 88, and 87%, respectively. Addition of Amiloride inhibited Na uptake in N cells by approximately 50% and completely suppressed the excess Na uptake in CRH cells to values observed in normal animals, i.e, 147 ± 33,224 ± 91, and 174 ± 86 cpm/1,000 cells in CRH cells at 1,3, and 120 min respectively, versus 181 ± 71,164 ± 57, and 238 ± 85 cpm/1,000 cells in N cells (p = NS at each time point). The results suggest that enhanced tubular cell growth in CRH is maintained from the in vivo setting to the in vitro cell culture system. Increased cellular Na transport in CRH is demonstrated to be via Na-H exchange by virtue of its reversion to normal in the presence of Amiloride. The results support the view that alterations in epithelial transport may be an important mechanism in the initiation or maintenance of cell growth.

          Related collections

          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1993
          1993
          12 December 2008
          : 64
          : 4
          : 615-620
          Affiliations
          Department of Medicine, University of Sydney, Concord Hospital, Sydney, Australia
          Article
          187410 Nephron 1993;64:615–620
          10.1159/000187410
          8366989
          © 1993 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 6
          Categories
          Original Paper

          Comments

          Comment on this article