Three new genera and eight new species of Hersiliidae are described from the East Palaearctic (Afganistan, Iran, Kazakhstan, Kyrgyzstan, Pakistan, Tajikistan, Turkmenistan, and Uzbekistan). The genus Hersiliola Thorell, 1870 (Araneae: Hersiliidae) is revised, and four new species are described. The genus includes nine species: H. afghanica Roewer, 1960 (Afghanistan); H. esyunini sp. n. (Uzbekistan); H. foordi sp. n. (Iran); H. lindbergi sp. n. (Afghanistan); H. macullulata (Dufour, 1831) (type species; from Spain and Algeria to Israel and Yemen); H. simoni (O.P.-Cambridge, 1872) (from Spain and Morocco to Israel); H. sternbergsi sp. n. (Turkmenistan, Uzbekistan); H. versicolor (Blackwall, 1865) (Cape Verde); and H. xinjiangenis (Liang & Wang, 1989) (Xinjiang, China). A new genus Duninia gen. n. is described, with two new species, Duninia baehrae sp. n. (type species; Turkmenistan) and D. rheimsae sp. n. (Iran). A new genus Deltshevia gen. n. is described, with two new species, Deltshevia danovi sp. n. (type species; Turkmenistan, Kazakhstan) and D. gromovi sp. n. (Uzbekistan, Kazakhstan). The widely ranging Central Asian Hersiliola pallida Kroneberg, 1875 (Kazakhstan, Kyrgyzstan, Pakistan, Tajikistan, Turkmenistan, Uzbekistan) is transferred to a new monotypic genus, Ovtsharenkoia gen. n.